妊娠早期小鼠子宫巨噬细胞分布和活性的变化  

Changes of macrophage distribution and activity in mouse uterine during early pregnancy

作　　者：贾永芳[1] 李莉[1] 宋兴丽[1] 杨梅[1] 张顺利[1] 

机构地区：[1]河南师范大学生命科学学院省部共建细胞分化调控国家重点实验室,新乡453007

出　　处：《解剖学杂志》2009年第1期49-51,共3页Chinese Journal of Anatomy

摘　　要：目的:通过研究妊娠早期子宫CD14+巨噬细胞数量、分布及酸性磷酸酶(ACP)活性变化,探讨巨噬细胞与母胎免疫耐受的关系。方法:用免疫组织化学方法和酶细胞化学研究小鼠妊娠早期子宫CD14+巨噬细胞及ACP的变化。结果:对照组CD14+巨噬细胞主要分布在子宫内膜。小鼠妊娠后内膜中数目减少,第3日最少;肌膜和外膜巨噬细胞增加,第2日达到高峰。CD14免疫反应阳性面积变化趋势与此类似。第3～5日期间ACP的活性下降并维持在较低的水平。结论:巨噬细胞在着床期抗原识别和处理能力受到了抑制,参与了妊娠早期母胎免疫耐受的形成。Objective： To study changes of the amount and distribution of mouse uterine CD14^＋ macrophage and expression of acid phosphatases （ACP） during early pregnancy and explore the role of macrophage in early pregnancy. Methods： The chan ges of CD14^＋ Mφand the activity of ACP were detected by the methods of immunochemistry and enzymocytochemistry. Results： In the control group, CD14^＋ Mφ were distributed dominantly in the endometrium. With increasing of gestational age, the amount of CD14^＋ Mφ in endometrium descented and reached the least in D3. CD14^＋ Mφ, were mostly located in myometrium and perimetrium of D2, and the amount was the most. The changes of positive area about CD14^＋ irnmunoreaction of macrophage in mouse uterine were in accordance with the amount. Compared with the control group, the content of ACP descented from D3 to D5 and kept in a low level. Conclusion： Abilities of recognized and disposed antigen of the macrophage were restrained in D3-DS. The macrophage may take part in materno-fetal immunotolerance in early pregnancy.

关 键 词：CD14 巨噬细胞 酸性磷酸酶 子宫 母胎免疫耐受 

分 类 号：R714[医药卫生—妇产科学]