离体大鼠缺血性心室颤动模型的建立  

Establishment of an isolated rat model of ischemia-induced ventricular fibrillation

作　　者：刘颖[1,2] 梁丹丹[2,3] 张弘[1,3] 刘懿[2,3,4] 肖俊杰[1,2] 陈义汉[1,2,3,4] 

机构地区：[1]同济大学附属同济医院心血管内科,上海200065 [2]同济大学心脏节律重点实验室,上海200065 [3]同济大学医学遗传研究所,上海200065 [4]同济大学医学院人类疾病基因研究中心,上海200065

出　　处：《同济大学学报（医学版）》2009年第1期20-22,共3页Journal of Tongji University(Medical Science)

基　　金：国家863计划项目(2007AA02Z438);国家973计划课题(2007CB512104);教育部创新团队计划项目(1508144002);上海市领军人才计划项目(LJRC0501);国家自然科学基金资助项目(30670872)

摘　　要：目的建立Langendorff灌流的离体大鼠缺血性心室颤动模型。方法80只健康Sprague-Dawley(SD)雄性大鼠,随机分为正常流量灌流组、正常流量灌流+burst刺激组、低灌流模拟心肌缺血组、低灌流模拟心肌缺血+burst刺激组,每组20只。采用Langendorff灌流装置行主动脉逆行灌流,通过缺血和短暂的burst刺激诱发心室颤动。结果正常流量灌流组、正常流量灌流+burst刺激组、低灌流模拟心肌缺血组心室颤动的发生率和持续率均为0%,而低灌流模拟心肌缺血+burst刺激组心室颤动的发生率为80%且持续率为50%(P<0.05)。结论在Langendorff心脏灌流系统中通过低灌流模拟心肌缺血,并给予burst刺激模拟应激事件,可以稳定地诱导心室颤动的发生。Objective To establish an isolated rat model of ischemia-induced ventricular fibrillation （VF）. Methods Eighty healthy Sprague-Dawley male rats were randomly divided into four groups including the normal perfusion group, the normal perfusion plus burst stimulation group, the low perfusion group and the low perfusion plus burst stimulation group. Burst stimulation was used during low perfusion to induce ventricular fibrillation in langendorff-perfused rat. Results The incidence of VF was 80% in the low perfusion plus burst stimulation group and 50% of that was persistent. However,no VF was induced in the other three groups （P 〈 0.05 ）. Conclusion VF can be stably induced by ischemia plus burst stimulation in langendorff-perfused rat.

关 键 词：心室颤动 缺血 模型 大鼠 

分 类 号：R541.75[医药卫生—心血管疾病] R331.38[医药卫生—内科学]