银杏叶总黄酮对胰岛素抵抗大鼠糖脂代谢和肝功能的影响  

Effects of total flavonoids in Gingko Biloba on glucose and lipid metabolism and liver function in rats with insulin resistance

作　　者：唐嘉航[1] 叶希韵[1] 刘江[1] 李萍[1] 张倩[1] 胡静婕[1] 

机构地区：[1]华东师范大学生命科学学院,上海200062

出　　处：《上海交通大学学报（医学版）》2009年第2期150-153,共4页Journal of Shanghai Jiao tong University:Medical Science

基　　金：华东师范大学本科生大夏科研基金(KY2007-078B)~~

摘　　要：目的探讨银杏叶总黄酮对胰岛素抵抗大鼠糖脂代谢及肝脏功能的影响。方法40只SD大鼠随机分为正常对照组、模型组、银杏叶总黄酮组和罗格列酮组(阳性药物对照组),每组10只。模型组、银杏叶总黄酮组和罗格列酮组大鼠以高糖、高脂饮食诱导建立胰岛素抵抗模型。分组处理12周后检测各组大鼠血糖、血脂水平和胰岛素抵抗、肝功能及肝脏抗氧化指标;肝脏组织脂肪染色观察组织学改变。结果银杏叶总黄酮组大鼠血糖水平、胰岛素抵抗指数(HOMA-IR)、胰岛素敏感指数(IAI)、血清总胆固醇(TC)、甘油三酯(TG)、肝脏丙二醛(MDA)含量以及血清转氨酶活力等均明显低于模型组(P<0.01);而高密度脂蛋白胆固醇(HDL-C)、血清总抗氧化能力(T-AOC)、肝脏超氧化物歧化酶(SOD)含量均显著高于模型组(P<0.01)。组织学观察显示,与模型组比较,银杏叶总黄酮组和罗格列酮组大鼠肝细胞内脂滴少且小。结论对于胰岛素抵抗大鼠,银杏叶总黄酮能有效调节血糖和血脂水平;增加胰岛素敏感性;减轻肝脏脂肪变性程度并改善肝功能,同时提高抗氧化能力。Objective To investigate the effects of total flavonoids in Gingko Biloba on glucose and lipid metabolism and liver function in rats with insulin resistance. Methods Forty SD rats were randomly divided into normal control group, model group, total flavonoids in Gingko Biloba group and rosiglitazone group （ positive drug control group） （ n = 10）. Models of insulin resistance were established by high glucose and high fat diet in model group, total flavonoids in Gingko Biloba group and rosiglitazone group. After treatment for 12 weeks, serum glucose, serum lipids, and parameters of insulin resistance, liver function and anti-oxidation capability were detected in each group, and histologic observations of liver tissues were conducted with adipose staining. Results The serum glucose, insulin resistance index （HOMA-IR） , insulin action index （IAI） , serum total cholesterol （ TC ） , triglyceride （TG） , liver malondialdehyde （MDA） and serum transaminase activity in total flavonoids in Gingko Biloba group were significantly lower than those in model group （P 〈0.01 ）, while high density lipoprotein-cholesterol （HDL）, serum total anti-oxidation capacity （T-AOC） and liver superoxide dismutase （SOD） were significantly higher than those in model group （ P 〈 0. 01 ）. Histologic observations revealed that there were smaller and less lipid droplets in liver cells in total flavonoids in Gingko Biloba group and rosiglitazone group than in model group. Conclusion Total flavonoids in Gingko Biloba can effectively regulate serum glucose and serum lipids levels, increase insulin sensitivity, decrease degree of adipose degeneration, and improve liver function and anti-oxidatin capacity in rats with insulin resistance.

关 键 词：银杏叶总黄酮 大鼠 胰岛素抵抗 脂肪肝 

分 类 号：R285[医药卫生—中药学]