大骨节病和骨性关节炎患者滑膜白介素-1β和肿瘤坏死因子-α的比较  

Expression of interleukin-1β and tumor necrosis factor-α in the synovium and synovial fluid of patients with Kashin-Beck disease and osteoarthritis

作　　者：孙正明[1] 凌鸣[2] 刘淼[1] 张银刚[1] 

机构地区：[1]西安交通大学医学院第一附属医院骨科,陕西西安710061 [2]陕西省人民医院骨科,陕西西安710068

出　　处：《南方医科大学学报》2009年第1期5-8,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(30400163)

摘　　要：目的比较大骨节病(KBD)和骨性关节炎(OA)关节滑膜和滑液中白介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)的表达,探讨两种病的发病机制。方法分别收集大骨节病和骨性关节炎患者关节滑膜和滑液20例和18例,以单纯半月板损伤患者的关节滑膜和滑液19例为对照;采用免疫组织化学染色法,观察大骨节病、骨性关节和半月板患者关节滑膜IL-1β和TNF-α阳性表达强度;采用ELISA法检测三种滑液标本IL-1β和TNF-α的水平。结果TNF-α在KBD和OA滑膜组织中的表达与对照组相比有显著性差异(P<0.05);IL-1β在KBD和OA滑膜组织中的表达与对照组相比也有显著性差异(P<0.01);IL-1β和TNF-α在KBD滑膜组织中的表达与OA相比无显著性差异(P>0.05)。TNF-α和IL-1β在KBD和OA滑液中的水平与对照组相比均有显著性差异(P<0.05);IL-1β和TNF-α在KBD滑液中的水平与OA相比均无显著性差异(P>0.05)。结论IL-1β和TNF-α可能在KBD和OA发生发展中扮演着重要的角色。Objective To investigate the role of interleukin-1β（IL-1β） and tumor necrosis factor-α （TNF-α） in the pathogenesis and progression of Kashin-Beck disease （KBD） and primary osteoarthritis （OA）. Methods The synovium and synovial fluid of the knee joint were collected from 20 adult patients with KBD, 18 with OA and 19 with meniscus injury （controls）. The expression of IL-1β and TNF-α in the synovium were analyzed by immunohistochemistry staining, and the levels of IL-1β and TNF-α in the synovial fluid were determined by enzyme-linked ir0munosorbent assay. Result IL-1β and TNF-α expressions in the synovium and their levels in the synovial fluid were significantly higher in patients with KBD and OA than in patients with meniscus injury （P〈0.05）, but comparable between KBD and OA groups （P〉0.05）. Conclusion IL-1β and TNF-α may play an important role in the pathogenesis and progression of KBD and OA.

关 键 词：大骨节病 骨性关节炎 滑膜 滑液 白介素-1Β 肿瘤坏死因子-Α 

分 类 号：R684.1[医药卫生—骨科学]