高氟低碘对Wistar大鼠大脑组织病理学及DNA损伤的影响  

High-Fluoride and Low-Iodine Induce Histopathological Changes and DNA Damage in the Brain of Offspring Rats

作　　者：洪建华[1] 牛瑞燕[2] 葛亚明[2] 王俊东[2] 

机构地区：[1]山西农业大学医院,太谷030801 [2]山西农业大学生态畜牧与环境兽医学山西省重点实验室,太谷030801

出　　处：《中国实验动物学报》2009年第1期10-14,I0002,I0003,共7页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金(编号:30170681)

摘　　要：目的研究高氟与低碘对子代大鼠大脑组织病理学及脑细胞DNA损伤的影响。方法断乳Wistar大鼠(雌∶雄=3∶1)32只,随机分为四组:对照组,饲喂大鼠标准饲料,去离子水;高氟组,饲喂大鼠标准饲料,饮100mg/L氟化钠(NaF)去离子水;低碘组,饲喂低碘饲料(定做),去离子水;高氟低碘组,饲喂低碘饲料,饮100mg/L氟化钠(NaF)去离子水。雌雄大鼠自然交配,待子代大鼠出生后,观察其在10、20天时脑皮质、海马组织病理学变化,及30、60和90天时脑DNA的损伤。结果与对照组相比,高氟组和低碘组大鼠大脑皮质及海马内有较多神经元核固缩、核溶解及细胞轮廓不清晰。高氟低碘组,有大量神经元出现核固缩、核溶解、锥体突明显拉长等现象,并且在大鼠发育期内尤其明显。随着日龄的增加,各实验组子代大鼠脑细胞DNA损伤程度显著增加,以高氟低碘组90日龄大鼠DNA损伤最严重。结论高氟与低碘影响了大鼠大脑皮质及海马组织形态结构与引起了脑细胞的DNA损伤。Objective To study the effects of high-fluoride and low-iodine on the histopathology and DNA in brain of offspring rats.Method Thirty-two Wistar rats were divided randomly into four groups,each of eight（female：male = 3：1）.The rats were treated with high-fluoride（100 mg NaF/L）,low-iodine（0.0855 mg/kg）,or both high-fluoride（100 mg NaF/L）and low-iodine（0.0855 mg/kg）,respectively.After mating,the offspring rats were born and used to assess the effects of the above described three factors on the structure of brain at postnatal day 10 and 20,and DNA damage at postnatal day 30,60 and 90.Results In comparison with the controls,the nuclei of many neurons were pyknotic or disappeared,the Nissl substance also showed various degrees of abnormality,and the dendrites were stretched in the high-fluoride and low-iodine groups,and in the high-fluoride plus low-iodine group,espacially in the early developmental stage.DNA damage in rat brain cells of the experimental groups was more serious than that in controls,especially in high-fluoride plus low-iodine group on postnatal day 90.Conclusion The results of this study demonstrate that the histopathological changes and DNA damage in the brain mainly occurred in the early stage of development after birth.Moreover,it may indicate that all those changes are adversely affected by exposure to high-fluoride,low-iodine,and both together in combination.

关 键 词：高氟低碘 子代大鼠 大脑 组织理学变化 DNA损伤 

分 类 号：Q959.837[生物学—动物学] S435.121.4[农业科学—农业昆虫与害虫防治]