五味子对实验性慢性心理应激下水上漂浮并高强度运动大鼠垂体-肾上腺皮质、性腺轴功能及糖代谢的影响  

Effects of Schisandra on the Function of the Pituitary-Adrenal Cortex,Gonadal Axies and Carbohydrate Metabolism in Rats Undergoing Experimental Chronic Psychological Stress,Navigation and Strenuous Exercise

作　　者：孙玲君[1] 王国洪[2] 吴波[3] 王坚[1] 王群[1] 胡兰萍[1] 邵加庆[1] 王扬天[1] 李洁[1] 顾萍[1] 卢斌[1] 

机构地区：[1]南京大学医学院临床学院/南京军区南京总医院内分泌科,江苏南京210002 [2]南京大学医学院临床学院/南京军区南京总医院放射免疫科,江苏南京210002 [3]南京大学医学院临床学院/南京军区南京总医院病理科,江苏南京210002

出　　处：《中华男科学杂志》2009年第2期126-129,共4页National Journal of Andrology

基　　金：南京军区"十一五"面上课题(06MA132)~~

摘　　要：目的:探讨五味子对实验性慢性心理应激下水上漂浮并高强度运动雄性大鼠垂体-肾上腺皮质、性腺轴功能及糖代谢的影响。方法:选取34只大鼠,随机分为非应激对照组(n=10)、应激对照组(n=12)和五味子组(n=12),应激对照组和五味子组参照Bedford的训练模式进行为期10d的训练,第11d进行3h的水上漂浮及3h的跑台高强度运动,实验结束后测定血清皮质酮(CORT)、T、LH、葡萄糖(Glu)水平。同时随机取一侧肾上腺在电子显微镜下观察其皮质的超微结构。结果:①应激对照组与非应激对照组相比:Glu水平升高,T水平降低(P<0.01),CORT水平无显著变化(P>0.05);灌服五味子组大鼠与应激对照组相比,Glu、CORT水平均显著降低(P<0.05);LH在3组之间均无显著变化(P均>0.05)。②电子显微镜观察发现,应激对照组肾上腺细胞内脂滴比五味子组减少并出现细胞凋亡。结论:五味子可降低应激大鼠血清CORT和Glu的水平,并在一定程度上保护肾上腺皮质细胞结构。Objective ： To investigate the effects of schisandra on the function of the pituitary-adrenal cortex, gonadal axies and carbohydrate metabolism in male rats undergoing experimental chronic psychological stress, navigation and strenuous exercise. Methods： Thirty-four SD rats were randomly allocated into a non-stress group （ Group A）, a stress control group （ Group B） and a schisandra group （Group C）. The latter two groups received 10 days of Benford＇s high-intensity training, followed by 3 hours of wearing floating with psychological stress and another 3 hours of running at the speed of 26.7 m/min. Then blood samples were immediately obtained for the measurement of the levels of testosterone （T）, corticosterone （CORT）, luteinizing hormone （LH） and blood glucose （Glu）. Meanwhile the adrenal gland was excised and its cortex uhrastructure observed under the electron microscope. Results ： The Glu level was increased while the T level decreased significantly in Group B as compared with Group A. The CORT level remained unchanged in Group B. Both the Glu and CORT levels were significantly reduced in Group C in comparison with B. However, no significant differences were found in serum LH levels among the three groups. And electron microscopy revealed a reduction of lipid droplets and apoptosis of the adrenal cortex cells in Group B as compared with C. Conclusion ： Scbisandra can reduce the levels of CORT and Glu and protect the structure of the adrenal cortex.

关 键 词：五味子 水上漂浮 高强度运动 睾酮 皮质酮 慢性心理应激 

分 类 号：R285[医药卫生—中药学]