单侧睾丸扭转后生精细胞凋亡的分子途径  

Molecular Pathways of Germ Cell Apoptosis Following Testicular Torsion in Rats

作　　者：刘子明[1] 郑新民[2] 

机构地区：[1]厦门大学附属中山医院泌尿外科,福建厦门361004 [2]武汉大学附属中南医院泌尿外科,湖北武汉430071

出　　处：《中华男科学杂志》2009年第2期144-148,共5页National Journal of Andrology

摘　　要：目的:研究大鼠单侧睾丸扭转复位后生精细胞凋亡的分子机制。方法:雄性SD大鼠16只,随机分为对照组和扭转组,每组8只。建立睾丸扭转动物模型(720°2h),术后24h留取手术侧睾丸。应用流式细胞术检测生精细胞凋亡和各级生精细胞计数,应用RT-PCR技术对Fas/FasL mRNA和Bax mRNA进行半定量分析,Western印迹技术检测细胞色素C含量。结果:两组间生精细胞凋亡及各类生精细胞计数均有显著性差异(P<0.01)。扭转组FCM直方图呈现高大凋亡峰,单倍体和四倍体细胞群计数下降,Fas/FasL mRNA和Bax mRNA表达上调,同时细胞质中细胞色素C含量亦明显升高,与对照组相比其差异均有显著性(P均<0.01)。结论:睾丸扭转复位后生精细胞凋亡存在着外源性和内源性两条基本途径。凋亡相关分子Fas/FasL表达上调和Bax介导的细胞色素C释放可能是睾丸扭转后生精细胞凋亡的重要环节。Objective： To investigate the molecular mechanism of germ cell apoptosis following testicular torsion in rats. Methods： Healthy male Sprague-Dawley rats （ n = 16 ） were equally randomized into a control and a torsion group and the models of testicular torsion （720° 2 h） were established. Twenty-four hours later, the apoptosis and count of germ cells were determined by flow cytometry, the expressions of Bax, Fas and Fas ligand （FasL） mRNA semiquantitatively analyzed by RT-PCR and the eytochrome C release detected by Western blot. Results ： Compared with the control group, there was an obvious increase in the number of apoptotic germ cells, a marked decrease in that of haploid and tetraploid cells and significantly up-regulated expressions of Bax and Fas/FasL mRNA in the torsion group （ P 〈0.01 ）. The Western blot analysis showed that the cytochrome C release was remarkably increased 24 hours after the detorsion. There were significant differences between the two groups （ P 〈 0.01 ）. Conclusion ： There are two major signaling pathways of cell apoptosis following testicular torsion, intercellular and intracellular. Up-regulated expressions of the apoptosis-related molecules Bax and Fas/FasL and increased cytochrome C release may play an important role in germ cell apoptosis following testicular torsion in rats.

关 键 词：睾丸扭转 生精细胞 凋亡 分子途径 

分 类 号：R697.22[医药卫生—泌尿科学]