长江口纹缟虾虎鱼繁殖季节的性腺组织学  

Histological studies on gonad of Tridentiger trigonocephalus(Gill) of the Yangtze Estuary in reproductive season

作　　者：章龙珍[1,2] 陈丽慧[1,2] 庄平[1,2,3] 冯广朋[1,3] 徐滨[1,3] 赵优[1,2] 

机构地区：[1]中国水产科学研究院东海水产研究所农业部海洋与河口渔业重点开放实验室,上海200090 [2]大连水产学院生命科学与技术学院,辽宁大连116023 [3]上海海洋大学水产与生命学院、上海市高校水产养殖学E-研究院,上海201306

出　　处：《水产学报》2009年第2期245-252,共8页Journal of Fisheries of China

基　　金：国家自然科学基金重大项目(30490234);国家科技基础条件平台项目(2004DKA30470-004);中央级公益性科研院所基本科研业务费专项(2008M01);上海市高校水产养殖学E-研究院建设项目(E03009);上海市长江口中华鲟自然保护区科研专项

摘　　要：采用常规组织切片法研究了长江口纹缟虾虎鱼各期卵巢和精巢的组织学结构特征。结果表明,每年的4、5、6月为长江口纹缟虾虎鱼的繁殖季节。进入繁殖季节的卵巢以Ⅳ期为主,4-6月的Ⅳ期卵巢分别占当月总数的62.32%、71.43%和48.41%。Ⅲ期卵巢只占当月总数的24.55%、12.33%、6.28%;Ⅴ期卵巢占13.13%、10.45%和26.67%;5、6月份Ⅵ期卵巢占5.89%、18.10%。Ⅲ-Ⅵ期的性腺指数（GSI）分别为4.49%±2.07%、31.75%±7.08%、38.33%±6.24%和7.78%±3.18%。4-6月精巢均处于Ⅳ、Ⅴ、Ⅵ期,4月以Ⅳ期为主,5、6月以Ⅴ期为主。Ⅳ-Ⅵ期精巢的GSI分别为0.66%±0.07%、0.96%±0.14%和0.43%±0.06%。纹缟虾虎鱼卵母细胞发育可以分为5个时相。Ⅲ期卵巢以第Ⅲ时相卵母细胞为主,这时期液泡、卵黄颗粒出现,同时含有少量Ⅰ时相和Ⅱ时相的卵母细胞;Ⅳ期卵巢以第Ⅳ时相卵母细胞为主,Ⅳ时相晚期卵母细胞开始出现油球,细胞核偏移和变形,放射带明显;Ⅴ期卵巢的卵细胞游离,卵膜外的二层滤泡膜脱落;Ⅵ期卵巢主要由第Ⅱ、Ⅲ时相卵母细胞和大量的空滤泡外膜组成。纹缟虾虎鱼精巢为小叶型,精巢内生殖细胞分为初级精母细胞、次级精母细胞、精细胞和精子,各期生殖细胞和支持细胞组成了精小囊,同一精小囊中的生精细胞发育同步。精子成熟后,精小囊破裂释放精子进入小叶腔,完成发育过程。The gonad of Tridentiger trigonocephalus （Gill） of the Yangtze Estuary in reproductive season was studied by light-microscope. The results showed that the reproductive season of T. trigonocephalus was April, May and June in every year. The ovaries in reproductive season were mostly in stage Ⅳ. From April to June, the ovaries in stage Ⅳ account for 62.32%, 71.43% and 48.41% of the total amount in every month respectively. The ovaries in stage Ⅲ account for 24.55%, 12.33% and 6.28% of the total amount in every month respectively. The ovaries in stage Ⅴ account for 13.13%, 10.45% and 26.67% of the total amount in every month respectively. The ovaries in stage Ⅵ account for 5.89% and 18.10% in May and June respectively. The corresponding gonadosomatic index （GSI） of ovaries from stage Ⅲ to Ⅵ were 4.49% ±2.07%, 31.75% ± 7.08%, 38.33% ±6.24% and 7.78% ± 3.18%. The testes were in stage Ⅳ, Ⅴ and Ⅵ from April to June. The testes were mostly in stage Ⅳ in April, in stage Ⅴ in May and June. The corresponding GSI of testes in stage Ⅳ, Ⅴ and Ⅵ were 0.66%±0.07%, 0.96% ± 0.14% and 0.43% ±0.06%. The oocytes could be divided into five phases. In the ovaries at stage Ⅲ, the oocytes were mostly at phase Ⅲ, follicular membrane and yolk grain appeared in the cytoplast. There were few oocytes at phase Ⅰ and Ⅱ at the same time. In the ovaries at stage Ⅳ, the oocytes were mostly at phase Ⅳ. The oil ball began to appear in the oocytes at the late phase Ⅳ. nucleus were excursion and distortional, zona radiate was clear at the same phase. In the ovaries at stage Ⅴ, the oocytes were dissociating. The two layers of follicular membrane which had outsided the plasma membrane were falling off. The ovaries at stage Ⅵ were mostly made of the oocytes at phase Ⅱ,Ⅲ and lots of discharged follicles. The testes of T. trigonocephalus were lobular type. All stages of spermatogenic cells were in the spermatogenic cysts. The wall of the spermatogenic cysts was made of sertoli cells. Sperma

关 键 词：纹缟虾虎鱼 卵巢 精巢 组织学 性腺指数 

分 类 号：S917[农业科学—水产科学]