河流弧菌对牙鲆表皮粘液的趋化作用  

Chemotactic response of Vibrio fluvialis to the skin mucus of Paralichthys olivaceus

作　　者：邹文政[1] 纪荣兴[1] 义家波[1] 鄢庆枇[1] 

机构地区：[1]集美大学水产学院福建省高校水产科学技术与食品安全重点实验室,福建厦门361021

出　　处：《水产学报》2009年第2期318-325,共8页Journal of Fisheries of China

基　　金：国家"八六三"高技术研究发展计划(2007AA09Z115);福建省科技项目(2006F5066);福建省科学基金项目(B0440002);厦门市海洋与渔业局科技项目(200707)

摘　　要：病原菌对粘液的趋化作用在其对粘液层定植过程中起着重要作用,趋化作用是病原菌的毒力机制之一。为了解病原性河流弧菌对牙鲆表皮粘液的趋化作用,本文采用改良的毛细管法研究了细菌浓度、孵育时间、孵育温度、pH、NaCl浓度、碳水化合物等对河流弧菌趋化作用的影响。试验结果表明,在较低浓度下,河流弧菌对牙鲆表皮粘液的趋化量随着菌浓度的升高而增大;在室温下河流弧菌的趋化量随着孵育时间的延长而增加,60 min趋于饱和;孵育温度在4-15℃范围内趋化作用随温度升高而增强,15℃时达最大值;pH为8时细菌的趋化性最强;NaCl浓度超过0.8%,河流弧菌的趋化性随着浓度升高而显著减弱（P〈0.05）;8种碳水化合物中甘露醇、乳糖、甘露糖能够极显著地促进河流弧菌对牙鲆表皮粘液的趋化作用（P〈0.01）。以上结果说明,牙鲆表皮粘液对河流弧菌有较强的趋化作用,该趋化作用受环境因子的影响较大;本文所揭示的河流弧菌趋化特性将对养殖牙鲆疾病防治提供有价值的参考。Chemotaxis of pathogen toward the mucus layer of fish plays an important role in its colonization in the mucus. Chemotaxis is considered to be one of the virulent mechanisms of pathogenic bacterium. For a better understanding of the chemotactic response of pathogenic Vibrio fluvialis toward the skin mucus of Paralichthys olivaceus so that preventive measures can be devised to reduce the bacterial colonization, the influences of bacteria concentration, incubation time, and environmental factors, such as temperature, pH, salinity and carbohydrate, on the bacterial chemotaxis were investigated by the method of modified capillary assay and isotope tracer. The results showed that the bacteria number of chemotactic V. fluvialis toward skin mucus of P. olivaceus increased with the bacteria concentration and incubation time, respectively. Saturation of bacterial chemotaxis was obtained after incubating at room temperature for 60 min; the chemotactic bacteria increased with the incubation temperature from 5℃ to 15℃ and peaked at 15℃, and optimal chemotaxis was observed at pH 8; the bacterial chemotaxis weakened with the increase of NaC1 concentration from 0. 8 % to 3. 6 %. Among 8 kinds of carbohydrate tested, mannitol, lactose and seminose promoted the bacterial chemotaxis remarkably. The results indicate that pathogenic V. fluvialis has a strong chemotaxis toward the skin mucus of P. olivaceus, and this chemotaxis is influenced remarkably by environmental factors, such as temperature, pH value, salinity and carbohydrate. The chemotaxis characteristics revealed in this paper would be helpful for the design of effective prophylactic strategies for P. olivaceus ＇ culture.

关 键 词：河流弧菌 牙鲆 表皮粘液 趋化作用 

分 类 号：Q579.1[生物学—生物化学] S917[农业科学—水产科学]