聚甲基丙烯酸甲酯/二氧化硅纳米义齿树脂材料遗传毒性的小鼠骨髓微核试验评价  

Genotoxicity evaluation of polymethyl methacrylate/SiO_2 denture nanocomposites by mouse bone marrow mirconucleus test

作　　者：阎立丹[1] 任勇武 王晨[3] 杜文鹏[3] 徐连来[3] 李长福[3] 

机构地区：[1]天津中医药大学第二附属医院口腔科,天津市300150 [2]河北省沧州市口腔医院修复科,河北省沧州市016000 [3]天津医科大学口腔医院修复科,天津市300070

出　　处：《中国组织工程研究与临床康复》2009年第3期479-482,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：天津市教委高等学校科技发展基金(020230)~~

摘　　要：背景:甲基丙烯酸甲酯/二氧化硅纳米复合材料已通过了包括细胞毒性试验、溶血试验、急性全身毒性试验、口腔黏膜刺激试验、皮肤致敏反应试验的生物安全性检测。目的:采用小鼠骨髓微核试验进一步探讨聚甲基丙烯酸甲酯/二氧化硅纳米义齿树脂对细胞遗传物质可能产生的损伤作用。设计、时间及地点:随机对照动物实验,于2007-11/2008-01在天津医科大学公共卫生学院毒理实验室完成。材料:SPF级昆明小白鼠50只,随机分为5组:阴性对照组,低、中、高剂量混悬液组,阳性对照组,10只/组。聚甲基丙烯酸甲酯/二氧化硅纳米义齿树脂由天津医科大学口腔医院制备。方法:将聚甲基丙烯酸甲酯/二氧化硅纳米义齿树脂材料粉碎成细小颗粒,过筛后加入生理盐水形成混悬液,质量浓度为0.3g/mL。阴性对照组灌胃生理盐水;低、中、高剂量混悬液组分别按15.0,7.5,3.75g/kg灌胃混悬液进行染毒;阳性对照组腹腔注射环磷酰胺,按40mg/kg给药。第1次染毒后24h再次染毒,末次染毒后6h取材,取胸骨骨髓加入胎牛血清制备涂片,Giemsa染色。主要观察指标:每只小鼠骨髓涂片计数1000个嗜多染红细胞,记录含微核的嗜多染红细胞数,求出微核率。结果:阴性对照组微核率为0.248%,阳性对照组为4.384%,低、中、高剂量混悬液组微核率分别为0.150%,0.254%,0.277%。与阴性对照组比较,低、中、高剂量混悬液组微核率无明显变化(P>0.05),阳性对照组微核率显著升高(P<0.01)。结论:聚甲基丙烯酸甲酯/二氧化硅纳米义齿树脂对小鼠细胞染色体无明显的诱变效应,具有较好的生物安全性。BACKGROUND： The polymethyl methacrylate （PMMA）/SiO2 denture nanocomposites have successfully passed the biological safety tests including cytotoxicity test, hemolysis test, acute systemic toxicity test, oral mucosa membrane irritation test and skin sensitization test. OBJECTIVE： To further investigate the possible impairment of PMMA/SiO2 denture nanocomposites on cellular germ plasm in mice by micronucleus test. DESIGN, TIME AND SETTING： A randomized control experiment was performed in the Toxicology Laboratory of Public Health College, Tianjin Medical University from November 2007 to January 2007. MATERIALS： Fifty healthy Kunming mice were divided into five groups： negative group, low-, middle-, high-dosage suspension groups, and positive group. The PMMA/SiO2 denture nanocomposites were prepared by Stomatological Hospital of Tianjin Medical University. METHODS： After grinding and sieving, the PMMA/SiO2 denture nanocomposites were added normal saline to prepare suspension, and the concentration was 0.3 g/mL. The mice in the negative group were administered normal saline. The mice in the low-, middle-, high-dose suspension groups were intragastdcally administered 15.0, 7.5, 3.75 g/kg suspension for toxicity. The mice in the positive group were administered （i.p.） 40mg/kg cyclophosphamide. At 24 hours after toxicity, the mice were administered again. At 6 hours after toxicity, the sternal marrow added into FBS to prepare for smears, and then Giemsa staining was performed. MAIN OUTCOME MEASURES： For each mouse, the number of micronucleated polychromatic erythrocytes （MNPCEs） in 1 000 polychromatic erythrocytes （PCEs） on the bone marrow smear was counted. The frequency of micronucleus was calculated in each group. RESULTS： The frequencies of micronucleus in the negative group, low-, middle-, high-dosage suspension groups, and positive group were 0.248%, 0.150%, 0.254%, 0.277% and 4.384%, respectively. Compared with negative group, the frequencies of micronucleus did not change obviousl

关 键 词：聚甲基丙烯酸甲酯 二氧化硅 微核 遗传毒性 

分 类 号：R318[医药卫生—生物医学工程]