小肠黏膜下层包绕丝素纤维复合韧带的生物力学测定  

Biomechanical properties of compound ligament with small intestinal submucosa wrapping silk fibers

作　　者：崔树北[1] 王洪[1] 杨述华[1] 杜靖远[1] 邵增务[1] 孟春庆[1] 段德宇[1] 文昌明[1] 王小红[1] 雷中华[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院骨科,湖北省武汉市430022

出　　处：《中国组织工程研究与临床康复》2009年第3期517-520,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：湖北省科技攻关计划(编号2006AA301C32)~~

摘　　要：背景:基于仿生化思想,综合丝素蛋白纤维和小肠黏膜下层的优点,利用小肠黏膜下层分层包绕丝素蛋白纤维,拟构建良好生物活性和力学性能的复合韧带。目的:检测小肠黏膜下层包绕丝素纤维复合韧带的生物力学特性。设计、时间及地点:对比观察实验,于2008-03/06在华中科技大学附属协和医院骨科实验室完成。材料:小肠取自封闭饲养的经过检疫的健康猪;蚕丝由湖北省德中茧丝有限公司惠赠。方法:用裹有纱布的刀柄去除小肠的浆膜层和肌层,制备符合要求的小肠黏膜下层。分层包绕脱胶后的蚕丝丝素纤维。直径分别为4,5,6,7,8,9mm,长度为15cm。将复合韧带置于AGS-H多功能材料实验机的夹具中,固定后以10mm/min的速度行拉断试验。主要观察指标:检测复合韧带的最大载荷,刚度和最大拉伸长度力学指标。结果:力学性能检测结果:复合韧带(以直径为7mm为例)的最大载荷,刚度和最大拉伸长度分别达到(2258±241)N,(309±16)N/mm和(43.0±1.5)%;而正常前交叉韧带的最大载荷,刚度和最大拉伸长度分别为2195N,306N/mm和33%。复合韧带和正常前交叉韧带的力学性质差异无显著性意义(P>0.05)。结论:复合韧带力学性能优秀,满足韧带组织工程支架材料力学要求。BACKGROUND： Take the advantage of silk fibers and small intestinal submucosa together, to construct a composite ligament, which has excellent biological activity and biomechanical properties, via the method of layered wrapping unglued silk fibroin fiber with small intestinal submucosa. OBJECTIVE： To detect the biomechanical properties of the small intestinal submucosa wrapping silk fibers of the ligament tissue engineering. DESIGN, TIME AND SETTING： A comparative observation experiment was performed at the Laboratory of Department of Orthopaedics, Affiliated Union Hospital of Huazhong University of Science and Technology from March to June 2008. MATERIALS： The small intestinal was obtained from healthy pigs, and the silk fibers were provided by Sino-Germany Silk Co., Ltd. METHODS： The placenta percreta and muscular layer of small intestine was removed to prepared small intestinal submucosa, which layered wrapping unglued silk fibroin fiber. Diameters were 4, 5, 6, 7, 8 and 9 mm, with length of 15 cm. The composite ligament was placed in fixture of AGS-H multi-purpose material testing machine, fixed, and performed abruption test at the speed of 10 mm/min. MAIN OUTCOME MEASURES： The mechanical properties, including maximum load, stiffness, as well as largest stretching length were detected. RESULTS： The results of mechanical properties test showed that the maximum load, stiffness and the largest stretching length of composite ligament （take a diameter of 7 mm for example） were （2 258±241 ） N, （309±16） N/mm and （43±1.5） %, respectively. And normal anterior cruciate ligament of the maximum load, stiffness and the largest stretching length were 2195 （N）, 306 （N/mm） and 33 （%）. There was no significant difference between two kinds of ligaments （P〉 0.05）. CONCLUSION： The excellent mechanical properties of composite ligament can meet the mechanical requirements of appropriate ligament tissue engineering scaffolds.

关 键 词：小肠黏膜下层 丝素纤维 韧带 生物力学 

分 类 号：R318[医药卫生—生物医学工程]