58S生物玻璃/壳聚糖复合多孔支架材料的制备  

Preparation of a 58S bioglass/chitosan composite porous scaffold

作　　者：谢林[1,2] 陈晓峰[1,2] 周嘉安[1,2] 赵娜如[1,2] 张姝江[1,2] 

机构地区：[1]华南理工大学材料学院生物材料研究所,广东省广州市510640 [2]华南理工大学特种功能材料教育部重点实验室,广东省广州市510640

出　　处：《中国组织工程研究与临床康复》2009年第3期525-528,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金项目(50672028;50702023;50830101)~~

摘　　要：背景:在前期研究的基础上用壳聚糖溶液与纯的58S生物玻璃支架进行复合,通过体外测试考察其生物活性,以确定是否符合组织工程对支架材料的要求。目的:观察58S生物玻璃/壳聚糖复合多孔支架的生物矿化特性及其与鼠骨髓间充质干细胞之间的体外相容性。设计、时间及地点:观察性实验,于2008-03/10在华南理工大学材料科学与工程学院生物材料研究所完成。材料:选用58S生物活性玻璃粉体为原料,利用预先处理过的聚氨酯泡沫作为模板,通过浸渍法制备58S生物玻璃支架基体,与壳聚糖复合后制成生物玻璃/壳聚糖复合支架。方法:①将试样放置于37℃恒温静态的100mL模拟生理溶液中,分别浸泡1,3,7,15d后取出样品,测试备用。②将第6代的小鼠骨髓间充质干细胞悬液以2×105/每个材料的密度接种到12孔培养板中的支架材料上复合培养。主要观察指标:采用X射线衍射分析和红外光谱分析方法对支架矿化不同时间表面生成的矿物进行分析和表征;利用扫描电子显微镜观察支架表面矿物生成情况和小鼠骨髓间充质干细胞在多孔支架材料上的生长情况。结果:①X射线衍射分析结果显示支架在模拟生理溶液中矿化7d后即出现标志羟基磷灰石形成的弱衍射峰,矿化15d后这些衍射峰变得更强,说明材料表面形成了低结晶度的羟基磷灰石。②红外光谱分析测试结果显示支架在矿化7d后即出现了标志羟基磷灰石形成的特征峰。③扫描电镜观察支架表面在矿化1d后有颗粒状的矿物生成,矿化15d后所形成的矿物成绒毛状,几乎覆盖整个支架表面。④小鼠骨髓间充质干细胞在支架材料上培养5d后黏附生长良好,进一步表明此支架材料具有较高的生物矿化特性和细胞相容性。结论:制备的58S/壳聚糖复合生物玻璃多孔支架具有较高的生物活性、与鼠骨髓间充质干细胞相容性良好。BACKGROUND： Chitosan composite with 58S bioglass scaffold was studied on the basis of prior work in this paper. The bioactivity of the scaffold was also studied through in vitro investigation to see whether it meets the request of tissue engineering OBJECTIVE： To investigate the bioactivity and the biocompatibility of the 58S/chitosan composite porous scaffolds with mouse bone marrow mesenchymal stem cells （BMSCs）. DESIGN, TIME AND SETTING： The observational study was performed at the Biomaterials Research Institute, College of Materials Science and Engineering, South China University of Technology from March to October 2008. MATERIALS： The porous bioglass 58S scaffold was prepared by immersion of PU foam with sol-gel derived bioglass powders. The composite scaffold was obtained with the further incorporation of chitosan. METHODS： Samples were placed in 100 mL of saline at 37 ℃, and immersed for 1, 3, 7 and 15 days, separately for use. At the sixth passage, mouse BMSCs were incubated on the composite scaffold in a 12-well culture plate at the density 2 ×10^5/material. MAIN OUTCOME MEASURES： X-ray Diffraction and Fourier Transform Infrared Spectroscopy was utilized to investigate the crystalline phase of the materials with different mineralizing time. Scanning Electron Microscopy was used to investigate the formation of the mineral on the surface and the growth of mouse BMSCs on the porous scaffold. RESULTS： From X-ray Diffraction pattern the weak diffraction peaks of hydroxyapatite could be seen after 7 days mineralization in simulated body fluid solution. The intensity of the diffraction peaks became stronger after 15 days mineralization. This showed that crystalline hydroxyapatite was formed on the scaffolds. Fourier Transform Infrared Spectroscopy results indicated that the characteristic peaks of hydroxyapatite appeared after 7 days mineralization. Scanning electron microscope observations showed that particular minerals were formed on the scaffold surface after 1 day mineralization. The

关 键 词：生物玻璃 壳聚糖 复合支架 羟基磷灰石 

分 类 号：R318[医药卫生—生物医学工程]