氯化铵对“黄海1号”中国对虾免疫相关酶类的影响  

Effect of ammonium chloride on immunity-related enzymes of "Huanghai No.1"population of shrimp Fenneropenaeus chinensis

作　　者：哈承旭[1,2] 刘萍[1] 何玉英[1] 李健[1] 李霞[2] 

机构地区：[1]农业部海洋渔业资源可持续利用重点开放实验室中国水产科学研究院黄海水产研究所,青岛266071 [2]大连水产学院,116023

出　　处：《渔业科学进展》2009年第1期34-40,共7页Progress in Fishery Sciences

基　　金：国家科技支撑计划(2006BAD01A13);国家自然科学基金项目(40706052);青岛市科技计划项目(07-2-3-5-jch)共同资助

摘　　要：在不同浓度氯化铵作用条件下对中国对虾"黄海1号"群体和中国对虾野生群体的存活率,血清中的溶菌酶(LSZ)、酚氧化酶(PO)、超氧化物歧化酶(SOD)、碱性磷酸酶(AKP)及酸性磷酸酶(ACP)活力变化进行了测定。发现"黄海1号"的24、48和72h的半数致死浓度(LC50)及安全浓度均高于野生群体;氯化铵浓度为8mg/L时,"黄海1号"体内的碱性磷酸酶、酸性磷酸酶活力极显著低于野生群体(P<0.01);氯化铵浓度为16mg/L时,"黄海1号"体内的溶菌酶活力显著高于野生群体(P<0.05),酸性磷酸酶活力显著低于野生群体(P<0.05);氯化铵浓度为32mg/L时,"黄海1号"体内的溶菌酶、酚氧化酶活力极显著高于野生群体(P<0.01),超氧化物歧化酶活力显著高于野生群体(P<0.05),酸性磷酸酶活力显著低于野生群体(P<0.05);氯化铵浓度为64mg/L时,选育群体"黄海1号"体内的超氧化物歧化酶活力显著高于野生群体(P<0.05);氯化铵浓度为128mg/L时,两群体的各项免疫指标都未出现明显差异。比较发现,随着氯化铵浓度的上升,选育群体中国对虾"黄海1号"几种与抗病力相关的生理指标都表现出了其比野生群体优良的特性。The survivorship and activity of lysozyme, phenoloxidase, superoxide dismutase, alkaline phosphatase and acid phosphatase in hemolymph of ＂Huanghai No. 1＂ and wild population of Fenneropenaeus chinensis, when they were exposed to different concentrations of Ammonia-N,were studied. The result showed that the 24hLC50, 48hLC50, 72hLC50 and safe concentration of ＂Huanghai No. 1＂ were higher than the wild population. When ammonium chloride was 8 mg/ L in the culture system, the alkaline phosphatase and acid phosphatase activities of ＂Huanghai No. 1＂were much lower than the wild population of F. chinensis （P〈 0.01）. When the concentration of ammonium chloride was 16 mg/L in the culture water, lysozyme activity of ＂Huanghai No. 1＂was higher than the wild population （P〈0.05） and alkaline phosphatase and acid phosphatase activity of ＂Huanghai No. 1＂was lower than the wild population （P〈0.05）. When the concentration of ammonium chloride was 32mg/L in the culture water, lysozyme and phenoloxidase activity of ＂Huanghai No. 1＂ was much higher （P〈0.01） and superoxide dismutase activity of ＂Huanghai No.1＂was higher （P〈0.05） than the wild population, and phosphatase and acid phosphatase activities of ＂Huanghai No. 1＂were lower than the wild population （P〈0.05）. When the concentration of ammonium chloride was 64mg/L in the culture water, superoxide dismutase activity of ＂Huanghai No. 1＂was much higher than the wild population （P〈0.01）. When the concentration of ammonium chloride was 128mg/L in the culture water, there were no obvious differences between the two populations. It was suggested that the anti-disease ability of selected ＂Huanghai No. 1＂ was better than the wild population at a range of low concentrations of Ammonia-N.

关 键 词：中国对虾黄海1号 氯化铵 溶菌酶 酚氧化酶 超氧化物歧化酶 

分 类 号：S948[农业科学—水产养殖]