氯胺酮、咪达唑仑及丙泊酚对发育期神经元细胞内钙的影响  

Effect of ketamine,midazolam and propofol on intracellular Ca^(2+) in developing neurons

作　　者：谭蕾[1] 罗爱林[1] 向强[1] 赵以林[1] 何璇[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院麻醉学教研室,湖北武汉430030

出　　处：《第四军医大学学报》2009年第5期405-407,共3页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金(30772086)

摘　　要：目的:探讨氯胺酮、咪达唑仑及丙泊酚对大鼠发育期海马神经元细胞内钙浓度的影响.方法:将原代培养第5日的大鼠海马神经元用10μmol/L的Ca2+指示剂Fluo-4共孵育30 min洗涤后,分别加入150μmol/L氯胺酮,咪达唑仑3μmol/L,丙泊酚10μmol/L,采用激光共聚焦显微镜选定多个细胞分别测定荧光强度的变化.结果:氯胺酮使体外培养第5日的海马神经元代表钙浓度的荧光强度明显下降[(987±307)vs(766±226),P<0.05],咪达唑仑,丙泊酚均使体外培养5 d的海马神经元荧光强度明显升高[(1707±514)vs(2663±572),(1057±353)vs(1749±708),P<0.05].结论:阻滞NMDA受体的氯胺酮降低发育期海马神经元细胞内钙浓度,而兴奋GABAA受体的咪达唑仑,丙泊酚则会升高细胞内钙浓度.AIM ： To investigate the effect of ketamine, midazo- lam and propofol on intraceflular Ca2＋ in developing primary hippoeampus neurons of rats. METHODS： On the fifth day, the cultured hippocampal neurons were co-incubated with 10 μmol/L Fluo-4 AM for 30 min at 37℃. Excess dye was removed with three rinses of DMEM. Fluorescence imaging of intracellular Ca2. was performed to detect a number of neurons selected when the hippoeampal neurons were exposed to 150μmol/L ketamine, 3 μmol/L midazolam or 10 μmol/L propofol respectively. RESULTS： After exposed to 150 μmolfL ketamine, the intracel- lular calcium concentration of the developing neurons decreased significantly and fluorescence intensity of the neurons decreased from （987 ± 307） to （766 ± 226） （P 〈 0.05 ）. When exposed to 3 μmol/L midazolam or 10 μmol/L propofol, the cytosolie Ca2 ＋of the neurons increased significantly and fluorescence intensity of the neurons exposed to midazolam increased from（ 1707 ± 514 ） to （2663 ± 572） （ P 〈 0.05 ）. Fluorescence intensity of the neurons exposed to propofol increased from（ 1057 ±353 ） to （1749 ±708） （ P 〈 0.05 ）. CONCLUSION ： Ketamine decreases the intracel- lular Ca2＋ of rat developing primary hippocampus neurons while midazolam and propofol may increase the intracellular Ca2 ＋ of the neurons.

关 键 词：氯胺酮 咪达唑仑 丙泊酚 原代培养 海马神经元 钙离子浓度 

分 类 号：R614.4[医药卫生—麻醉学] R614.2[医药卫生—外科学]