异种骨移植材料制备及其骨诱导活性实验研究  

PREPARATION OF XENOGENEIC BONE GRAFT AND ITS OSTEOINDUCTIVITY

作　　者：陈学英[1,2] 李宝兴[1,2] 李靖[2] 殷少芳[2] 宋会平[3] 原林[1] 

机构地区：[1]南方医科大学解剖学教研室,广州510515 [2]山西省医用组织库 [3]唐山市第二医院创伤科

出　　处：《中国修复重建外科杂志》2009年第3期362-365,共4页Chinese Journal of Reparative and Reconstructive Surgery

摘　　要：目的采用酶处理法去除猪骨组织中的主要异种抗原——α-半乳糖抗原(α-Gal),探讨不同处理方式对其力学性能和骨诱导活性的影响。方法取新鲜猪髂骨用α-半乳糖苷酶处理去除α-Gal,冷冻干燥后辐照灭菌。根据处理方法不同,将猪髂骨分为:A组新鲜骨、B组去抗原骨、C组冻干去抗原骨及D组冻干辐照去抗原骨。取B组骨扫描电镜观察,SmileView软件测量骨髓腔大小;采用ELISA法检测A、B及D组骨块α-Gal含量;检测A、B、C及D组力学性能;将去抗原脱矿骨(实验组)及脱矿骨(对照组),植入Wistar大鼠股部肌袋,于术后3、4、5、6周取出植入骨行组织学观察及ALP活性检测。结果制备的B组骨呈三维网状结构,与人松质骨类似,骨髓腔大小为150～600μm。A、B、D组α-Gal吸光度值分别为0.358±0.027、0.191±0.011和0.190±0.009,组间差异有统计学意义(P<0.05)。A、B、C、D组间力学性能比较差异无统计学意义(P>0.05)。实验组植入3周,骨内长入的间充质细胞转变成软骨细胞并开始分泌软骨基质,4周骨内形成软骨组织,6周骨边缘成骨活跃,形成类骨质,骨内可见成熟软骨组织;对照组植入3～6周显示植入骨吸收,未见成骨迹象。实验组植入3～6周的ALP活性均显著高于对照组,差异有统计学意义(P<0.05)。结论酶处理法能有效去除猪骨组织中的主要异种抗原,保留了其力学性能与骨诱导活性,有望成为一种骨移植替代材料。Objective To develop a deantigenated porcine bone graft through enzyme treatment and to study the biomechanical properties and osteoinductivity of the xenogeneic bone graft. Methods Deantigenated xenogeneic bone was prepared from porcine bone by a series treatment including α-galactosidase （α-Gal） treatment,freezedrying and irradiation at a does of 25 kGy. Samples were divided into 4 groups according to the treatment methods： fresh bone （group A）; deantigenated bone （group B）; deantigenated and freezedried bone （group C）; deantigenated-freezedried-irradiated bone （group D）. SEM observation to group B samples was performed and the diameters of the caves of the porcine cancellous bone were measured. The α-Gal contents of samples of groups A,B,D were measured by ELISA. Theffe ects of di erent treatments on porcine bone mechanic properties wereffevaluated through compressive test of 4 groups. The prepared porcine cortical bone were demineralized and ectopically implanted into muscle groups of hind limbs of Wistar rats as experimental group,and the demineralized cortical bone which were inacitviated by high temperature and pressure were implanted as control group. Histological observation was performed and ALP activity was tested at di erent time postoperatively to investigate the osteoinductivity of the xenogeneic bone implants. Results The porous structure of prepared porcine cancellous bone was similar to that of human cancellous bone. The diameters of the caves were between 150-600 μm. The A value of the groups A,B,D was 0.358 ± 0.027,0.191 ± 0.011,0.191 ± 0.009,respectively,with statistically di erences between groups （P 〈 0.05）. While the biomechanical properties among 4 groups had no statistically di erence （P 〉 0.05）. Histological observation showed mesenchymal cells immigrated into cartilage and converted into chondrocytes at 3 weeks postoperatively. Cartiliage was formed at 4 weeks and osteoid and more adult cartilage was formed at 6 weeks within the implants of exper

关 键 词：异种骨移植 去抗原 力学性能 骨诱导活性 

分 类 号：R318.08[医药卫生—生物医学工程] R687.3[医药卫生—基础医学]