小麦胚乳细胞内、细胞间胞质骨架分布格局的超微结构观察  

ULTRASTRUCTURAL OBSERVATIONS ON THE DISTRIBUTION OF THE INTRA AND INTERCELLULAR CYTOSKELETON OF THE ENDOSPERM CELLS IN TRITICUM AESTIVUM 

作　　者：张伟成[1] 李瑞秋 严文梅[1] 高小彦[1] 

机构地区：[1]中国科学院上海植物生理研究所

出　　处：《Acta Botanica Sinica》1998年第4期298-302,共5页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家自然科学基金"八五"重点项目

摘　　要：以小麦（ＴｒｉｔｉｃｕｍａｅｓｔｉｖｕｍＬ．）幼嫩胚乳为材料，经ＴｒｉｔｏｎＸ１００抽提、ＤＧＤ（ｄｉｅｔｈｙｌｅｎｅｇｌｙｃｏｌｄｉｓｔｅａｒａｔｅ）渗透、包埋，制备去包埋剂超薄切片，对细胞内、细胞间胞质骨架的分布格局与特征进行了电镜观察。由所获图像可见，胞质骨架呈主要由微管、微丝组成的三维网络结构；特别值得注意的是，有不少５～７ｎｍ的微丝在多处从网络表层向胞壁界面方向突出，并时而可见其横贯分界壁连接相邻骨架网络而将相邻细胞骨架联成一体。胚乳组织中微丝的跨胞分布以两种形式存在，直径达１００～２００ｎｍ微丝束的跨越和单个微丝的分散贯穿，看来这与该组织中开放态胞间通道与正常胞间连丝同时并存相吻合。初步讨论了微丝参与正常胞间连丝结构的可能性。By means of Triton X 100 extraction and DGD (diethylene glycol distearate) embedment free section method the distribution pattern and characteristics of intra and intercellular cytoskeleton of endosperm cells of Triticum aestivum L. were studied with electron microscopy. Three dimensional architecture of the cytoskeleton could be recognized as a meshwork mainly composed of microtubules (MT) and microfilaments (MF). Attention was stressed on the interface of the adjoining cytoskeletal frameworks where an attractive phenomenon observed was that the MF extruding from the surface of the cytoskeleton often traversed the whole wall boundary and connected the neighbouring frameworks into an entity. In the endosperm tissue two types of transcellular MF distribution could be distinguished, the MF in bundles traversing the enlarged intercellular channels and the MF individually penetrating the wall boundary;that seemed to coordinate with the co presence of normal and modified plasmodesmata in the same wall. The above observations demonstrated the intercellular cytoskeletal continuity within the symplast and confirmed that the MF was the main constituent of the traversing cytoplasmic strands, the possibility of MF being organized as a structural element of the normal plasmodesmata was also discussed.

关 键 词：胞质骨架 胚乳 微丝 胞间连丝 小麦 超微结构 

分 类 号：Q944.59[生物学—植物学]