白细胞介素-10单核苷酸多态性与呼吸道合胞病毒毛细支气管炎的病例-对照研究  

Case-Control Study on Correlation between Interleukin-10 Single Nucleotide Polymorphisms and Respiratory Syncytial Virus Bronchiolitis

作　　者：董琳[1] 黄志英[1] 陈小芳[1] 周晓聪[1] 李锦燕[1] 林剑[1] 张海邻[1] 

机构地区：[1]温州医学院附属育英儿童医院呼吸科,浙江温州325027

出　　处：《实用儿科临床杂志》2009年第4期268-270,共3页Journal of Applied Clinical Pediatrics

基　　金：浙江省医药卫生科研项目资助(2004B148)

摘　　要：目的探讨温州地区汉族儿童IL-10/T-819C和A-1082G位点单核苷酸多态性(SNPs)的分布特征及其与呼吸道合胞病毒(RSV)毛细支气管炎易感性、病情的关联和对血清总IgE和鼻咽分泌物(NPS)IL-10的影响。方法采用DNA测序法检测114例汉族RSV毛细支气管炎患儿(病例组)和90例健康儿童(健康对照组)IL-10/T-819C、A-1082G位点SNPs;应用化学发光法检测病例组血清总IgE;用ELISA法检测病例组NPS IL-10水平。结果病例组IL-10/T-819C位点TT、CT、CC基因型频率比较分别为45.6%、40.4%和14.0%,等位基因频率分别为T65.8%、C34.2%;健康对照组基因型频率分别为TT47.8%、CT36.7%、CC15.5%,等位基因频率分别为T66.1%、C33.9%,二组基因型及等位基因频率比较差异均无统计学意义(χ2=0.306,0.005 Pa>0.05)。病例组IL-10/A-1082G AA、AG基因型频率分别为86.8%、13.2%,等位基因频率分别为A93.4%、G 6.6%;健康对照组基因型频率分别AA87.8%、AG12.2%,等位基因频率分别为A93.9%、G6.1%,二组比较差异均无统计学意义(χ2=0.040,0.037 Pa>0.05)。病例组IL-10/T-819C和IL-10/A-1082G位点不同基因型患儿NPS IL-10和血清总IgE水平比较差异均无统计学意义(H=0.676,F=0.687;Z=0.620,t=1.107 Pa>0.05)。IL-10/T-819C位点和A-1082G位点基因型频率在轻度和中重度患儿之间的差异均无统计学意义(χ2=0.779,1.793 Pa>0.05)。结论温州地区汉族儿童存在IL-10/T-819C和A-1082G位点基因多态性,但未发现其与RSV毛细支气管炎存在关联。Objective To explore the distribution characteristics of the single nucleotide polymorphisms （SNPs） of IL - 10 gene in Hart children in Wenzhou area, and the association of SNPs of IL- 10/T- 819C and A - 1082G with the susceptibility to respiratory syncytial virus （RSV） bronchiolitis and disease severity, and to explore the impact of the SNPs upon total serum IgE levels and IL- 10 production in nasopharyngeal secretions （NPS） in children with RSV bronchiolitis. Methods One hundred and fourteen children （ patients group） admitted to hospital with RSV bronchiolitis and 90 healthy children （control group） were recruited. Sequence analysis was used to detect the SNPs of IL - 10/T - 819C and A - 1082G. Total serum IgE levels in patients group were assayed with ELISA and NPS IL - 10 levels were detected with chemiluminescence assay. Results The genotype frequency of IL - 10/T - 819C was TT 45.6%, CT 40.4% , CC 14.0% in patients group,and TT 47.8% ,CT 36.7% ,CC 15.5% in the control group,it showed no significant difference between 2 groups （x^2 =0. 306 P 〉 0.05）. The frequency of each allele was T65.8% ,C 34.2% in patient group,and T66.1% ,C 33.9% in the control group,there was no significant difference in allele frequency between 2 groups （ x^2 = 0. 005 P 〉 0.05 ）. The genotype frequency of IL - 10/A - 1082G was 86.8 % （AA）, 13.2% （AG） in patients group, and AA 87.8%, AG 12.2% in the control group, it showed no significant difference between 2 groups （ x^2 = 0. 040 P 〉 0. 05）. The frequency of each allele was A 93.4%,G 6.6% in patients group,and A 93.9%,G 6. 1% in the control group, the difference was not significant between 2 groups （ x^2 = 0. 037 P 〉 0.05 ）. Furthermore,no differences were found either in total serum IgE or NPS IL - 10 levels among different genotype of T - 819C and A - 1082G in patients group （ H = 0. 676, F = 0. 687 ; Z = 0. 620, t = 1. 107 Pa 〉 0.05 ）. The differences in genotype frequency of IL - 10/T - 819C and IL - 10/A - 1082G were not

关 键 词：呼吸道合胞病毒 毛细支气管炎 基因多态性 白细胞介素-10 病例-对照研究 

分 类 号：R725.6[医药卫生—儿科]