支气管哮喘大鼠血管内皮生长因子与γ-干扰素、白细胞介素-4变化的相关性及布地奈德干预对其的影响  

Relationships of Vascular Endothelial Growth Factor and Interferon-γ,Interleukin-4 in Asthmatic Rats and Effect of Budesonide on Their Expression

作　　者：金小红[1] 李昌崇[2] 陈存国[1] 李绍波[1] 李凤仙[1] 干灵红[3] 

机构地区：[1]温州医学院附属台州医院儿内科,浙江临海317000 [2]温州医学院附属育英儿童医院呼吸科,浙江温州325027 [3]温州医学院附属台州医院中心实验室,浙江临海317000

出　　处：《实用儿科临床杂志》2009年第4期282-284,共3页Journal of Applied Clinical Pediatrics

基　　金：台州市科技局项目资助(051ky16)

摘　　要：目的探讨血管内皮生长因子(VEGF)、IFN-γ、IL-4在急性哮喘大鼠模型中的表达变化及其相关性及布地奈德混悬液干预后的变化。方法清洁级雄性SD大鼠36只随机分为对照组、哮喘组和布地奈德干预组。实验第1天和第8天,哮喘组和布地奈德干预组大鼠腹腔注射鸡卵清蛋白(OVA)/氢氧化铝混合液致敏2次,第15天开始给予大鼠喷雾(10 g/L OVA,30 min/d),连续激发2周,制备哮喘模型。对照组致敏和激发均以9 g/L盐水替代OVA。布地奈德干预组于每次抗原激发前0.5 h予布地奈德雾化吸入,12 h后相同剂量再雾化1次,共2周。各组大鼠末次激发24 h后处死,收集血液与支气管肺泡灌洗液(BALF)。应用ELISA法测定其血清和BALF中IL-4、IFN-γ和VEGF水平。结果哮喘组和布地奈德干预组大鼠BALF和血清IL-4水平均显著高于对照组(P<0.01,0.05);布地奈德干预组BALF和血清IL-4水平均显著低于哮喘组(Pa<0.01)。哮喘组BALF和血清IFN-γ水平均显著低于对照组和布地奈德干预组(Pa<0.01)。哮喘组BALF和血清VEGF水平均显著高于对照组和布地奈德干预组(Pa<0.01);布地奈德干预组血清和BALF中VEGF水平与对照组比较均无显著差异。BALF中IL-4与VEGF水平呈显著正相关(r=0.818 P<0.01),IFN-γ水平与VEGF水平呈显著负相关(r=-0.646 P<0.01);血清IL-4水平与VEGF水平呈显著正相关(r=0.690 P<0.01),IFN-γ水平与VEGF水平呈显著负相关(r=-0.479 P<0.01)。结论哮喘急性发作时存在Th1/Th2失衡,且Th1/Th2失衡与VEGF的表达存在明显的相关性,布地奈德雾化吸入可调节Th1/Th2的平衡及抑制VEGF的表达。Objective To explore the relationship of vascular endothelial growth factor（ VEGF）, IFN - γ and IL - 4 and effect of Budesonide on their in asthmatic rats. Methods Thirty - six male SD rats were randomly divided into 3 groups ： asthma group, Budesonide - treatment group and control group. On the first day of the experiment and the 82 day, the rat models of the asthma group and Budesonid treatment group were allergized by the OVA/Al（ OH）3 through intraperitoneal injection, respectively. And starting from the 15^th day, they were challenged by the OVA through atomization for 2 weeks. Control group was allergized and challenged by NS atomization. Budesonid treatment group was interfered in Budesonide inhalation before suscitation in O. 5 h. After 12 h the same inhal done was again in Budesonide group. Twenty - four hours after the last challenge, the rats in 3 groups were sacrificed, and blood and bronchoalveolar lavage fluid （BALF） were collected. The concentrations of IL - 4,IFN - γ and VEGF in serum and BALF were measured by enzyme - linked immunosorbent assay. Results The con- centrations of IL -4 in serum and BALF in asthma group and Budesonide treatment group were significantly increased than those in control group（ P 〈 0.01,0.05 ） , respectively ; the concentrations of IL - 4 in serum and BALF in Budesonide treatment group were significantly decreased than those in asthma group（ P, 〈 0.01 ）. The concentrations of IFN -γ in serum and BALF in asthma group were significantly decreased than those in control group and Budesonide treatment group （ Pa 〈 0.01 ）. The concentrations of VEGF in serum and BALF in asthma group were significantly increased than those in control group and budesonide treatment group （ Pa 〈 0.01 ） , there were no significant difference between control group and budesonide treatment group. In BALF,there was a significant positive correlation between the concentrations of IL - 4 and VEGF （r = 0. 818 P 〈 0.01 ） and a significant negative correlation

关 键 词：哮喘 血管内皮生长因子 Γ-干扰素 白细胞介素-4 布地奈德 大鼠 

分 类 号：R725.6[医药卫生—儿科]