幽门螺杆菌cagA、vacA、iceA基因与儿童结节性胃炎的关系  

CagA,VacA and IceA Genotypes of Helicobacter Pylori and Nodular Gastritis in Children

作　　者：周颖[1] 黄瑛[1] 邵彩虹[1] 张冰峰[1] 王小卉[1] 

机构地区：[1]复旦大学附属儿科医院消化科,上海201102

出　　处：《中国临床医学》2009年第1期63-66,共4页Chinese Journal of Clinical Medicine

基　　金：上海市自然科学基金项目(06ZR14017)

摘　　要：目的:探讨幽门螺杆菌(Hp)cagA、vacA、iceA基因与儿童结节性胃炎的关系。方法:收集2007年5月—2008年1月行胃镜检查确诊Hp感染的22例结节性胃炎和22例非结节性胃炎患儿的胃粘膜组织,分别用聚合酶链反应(PCR)检测cagA、vacA和iceA基因,病理检查了解胃窦粘膜炎症程度。结果:结节性胃炎组中cagA基因单独检出率为68.2%,vacAs1/m1单独检出率为13.6%,vacAs1/m2单独检出率为45.5%,iceA1单独检出率为72.7%,iceA2单独检出率为18.2%,非结节性胃炎组中cagA基因单独检出率为68.2%,vacAs1/m1单独检出率为22.7%,vacAs1/m2单独检出率为27.3%,iceA1单独检出率为63.6%,iceA2单独检出率为22.7%。不同基因型菌株在结节性胃炎和非结节性胃炎中的检出率无统计学意义(P>0.05)。在轻度、轻-中度、中度慢性结节性胃炎中,cagA型菌株检出率分别为66.7%、50%、75%,vacAs1/m1型菌株检出率分别为11.1%、0%、25%,vacAs1/m2型菌株检出率分别为55.6%、25%、37.5%,iceA1型菌株检出率分别为88.9%、50%、75%,iceA2型菌株在轻度、轻-中度、中度慢性结节性胃炎中的检出率分别为0%、50%、25%,不同基因型菌株与结节性胃炎胃窦粘膜炎症的严重程度无关(P>0.05)。结论:cagA、vacA、iceA基因与结节性胃炎、胃粘膜的炎症严重程度无关,提示除菌株因素外,宿主因素、环境因素在结节性胃炎的形成过程中可能发挥了重要作用。Objective： To investigate the relationship between cagA, vacA, iceA genotypes of Helicobacter pylori（Hp） and nodular gastritis in children . Methods：From May 2007 to January 2008, 22 children were diagnosed as Hp infected and nodular gastritis by endoscopy. Biopsy specimens were taken from their gastric antrum with one for polymerase chain reaction（PCR） for detecting cagA, vacA and iceA genes and two for histological examination. Results： Among nodular gastritis group, single cagA was detected in 68.2% of isolates, vacA s1/ml and vacA s1/m2 were present in 13.6% and 45.5% of isolates, respectively, iceA1 and iceA2 were found in 72.7% and 18.2% of isolates, respectively. Among non-nodular gastritis group, single cagA was detected in 68.2% of isolates, vacA sl/ml and vacA s1/m2 were present in 22.7% and 27.30/oo of isolates, respectively, iceA1 and iceA2 were found in 63.6% and 22.7% of isolates, respectively. There was no statistical correlation in geno types between the two group, cagA was detected in 66.7%, 50%, 75% of isolates from patient with mild, mild to moderate, moderate chronic gastritis, respectively, vac s1/m1 was detected in 11. 1%, 0%, 25% of isolates, respectively, vac s1/m2 was detected in 55.6%, 25%, 37.5% of isolates, respectively, iceA1 was detected in 88.9%, 50%, 75% of isolates, respectively, iceA2 was detected in 0%, 50%, 25% of isolates, respectively. No association was observed between Hp genotypes and the degree of imflammation in nodular gastritis. Conclusion： This study suggest the potential role of host and environment factors for the development of clinical disease at a later age.

关 键 词：幽门螺杆菌 结节性胃炎 基因 

分 类 号：R725.7[医药卫生—儿科]