微卫星在种公牛个体识别与亲缘鉴定方面的应用  

Individual identification and paternity testing of bulls using microsatellite

作　　者：王静[1] 刘丑生[2] 张利平[3] 王志刚[2] 于福清[2] 张桂香[2] 孙秀柱[2] 韩旭[2] 魏玉春[4] 

机构地区：[1]兰州职业技术学院,兰州730070 [2]农业部全国畜牧总站畜禽牧草种质资源保存利用中心,北京100193 [3]甘肃农业大学动物科学技术学院,兰州730070 [4]西北农林科技大学动物科技学院,杨凌712100

出　　处：《遗传》2009年第3期285-289,共5页Hereditas（Beijing)

基　　金：农业部畜禽牧草品种资源保护项目(编号:农财发[2007]43号)资助

摘　　要：采用美国ABI公司牛亲子鉴定试剂盒(Bovine Paternity PCR Typing Kit,包括11个常染色体)和3个自选的Y染色体微卫星座位,检测我国部分种公牛站肉用种公牛14个微卫星座位的多态性分布,评估其遗传多样性,并探讨其用于个体识别与亲缘鉴定的可行性。结果表明:种公牛在14个微卫星座位中遗传多样性均较高,其中MCM158座位的平均多态信息含量最高达到0.888,ETH10座位的最低,为0.482。单个座位的个体识别能力在0.715～0.968之间,累积个体识别能力为99.99%,累计非父排除率达到99.99%,表明采用的14个位点适用于个体识别和亲缘鉴定。The polymorphism distributions of 14 microsatellite loci were detected using the Bovine Paternity PCR Typing Kit （includingl 1 X-STR） and 3 selected Y-STR microsatellite DNA markers. The genetic diversity were evaluated, and the feasibility of the application to individual identification and paternity testing were discussed. The results showed that all the 14 microsatellite loci had genetic polymorphisms in bulls, and the polymorphism information content （PIC） in loci MCM158 was the biggest （0.888）, while the ETHIO was the lowest （0.482）. Power of discrimination （DP） value of the 14 STR loci ranged from 0.715 to 0.968. The Cumulate DP （CDP） was 99.99%, and the Cumulate PE （CPE） also reached 99.99%. These results indicate that the 14 microsatellites can be applied to the individual identification.

关 键 词：种公牛 微卫星 个体识别 亲缘鉴定 

分 类 号：S823[农业科学—畜牧学] Q953[农业科学—畜牧兽医]