基于线粒体ND1和16SrRNA基因序列探讨中国蝴蝶12科的系统发育关系(鳞翅目：双孔次亚目：蝶类)  

Molecular phylogeny of 12 families of the Chinese butterflies based on mitochondrial ND1 and 16S rRNA gene sequences(Lepidoptera:Ditrysia:Rhopalocera)

作　　者：邹方振[1] 郝家胜[1] 黄敦元[1,2] 张大秀[1] 朱国萍[1] 朱朝东[2] 

机构地区：[1]安徽师范大学生命科学学院分子进化与生物多样性实验室,安徽芜湖241000 [2]中国科学院动物研究所动物进化与系统学所级重点实验室,北京100101

出　　处：《昆虫学报》2009年第2期191-201,共11页Acta Entomologica Sinica

基　　金：安徽省优秀青年基金(08040106811);安徽省“重要生物资源保护与利用”重点实验室及中青年学术带头人专项基金(No.590620);安徽师范大学生物大分子重点实验室开放课题基金(2006A001);安徽省高校“生物环境与生态安全”省级重点实验室专项基金资助课题

摘　　要：对中国12科共32种代表蝶类的ND1基因和16S rRNA基因进行了序列测定(包括新测30种ND1基因和9种16S rRNA基因)和比较分析,同时采用邻接法、最大似然法和贝叶斯法构建了12科蝶类的系统发育树,探讨了其高级分类群的系统发育关系。序列分析的结果显示:经比对处理后的两个基因总长度为869 bp,其中保守位点373个,可变位点496个,简约信息位点375个;A+T的平均含量为80.2%,明显高于C+G的平均含量19.8%。分子系统树表明:蛱蝶科不是单系群;珍蝶类、斑蝶类和喙蝶类位于蛱蝶科内;粉蝶科和凤蝶科具有共同祖先。据此建议:绢蝶科应归入凤蝶科;蚬蝶科归入灰蝶科;珍蝶类、斑蝶类和喙蝶类作为蛱蝶科中的亚科,眼蝶类从蛱蝶科中分离出来独立成科。另外,环蝶类的系统分类地化还有待于进一步研究。In this study, the phylogenetic relationships of 12 families of the Chinese butterflies were investigated based on mitochondrial ND1 and 16S rRNA gene sequences. Thirty-two Chinese species were included in the analyses, of which 30 species were sequenced for the ND1 and 9 for 1he 16S rRNA gene. In the alignment, the two combined genes were 869 bp in length, including 373 conserved, 496 variable and 375 parsimonious-informative sites, respectively. The average percentage of A ＋ T is 80.2%, which is much higher than that of C ＋ G （19.8%）. With both Zygaena angelicae and Z. transalpina as the outgroup taxa, the phylogenetic trees were reconstructed with the neighbor-joining （NJ）, maximumlikelihood （ML） and Bayesian inference （BI） methods. Major results included that： 1 ） Nymphalidae is not monophyletic; 2） the acraeids, danaids and libytheids are included in Nymphalidae; 3 ） the Pieridae and Papilionidae have a closest common ancestor; 4） the Parnassiidae should be included in Papilionidae; and 5 ） the satyrids should be separated from the Nymphalidae and be given a family rank. In addition, our results also suggest that the Riodinidae should be classified into Lycaenidae, and the acraeids should be given a subfamily rank. As to the amathusiidae, its taxonomic level and phylogentic position need further more detailed studies.

关 键 词：鳞翅目 双孔次亚目 蝶类 ND1基因 16S RRNA基因 系统发育 

分 类 号：Q969[生物学—昆虫学]