EPO上调人CD34^＋脐血干细胞CD26的表达  

Erythropoietin induces CD26 upregulation in CD34^+ human cord blood hemato-poietic cells

作　　者：范娅涵[1] 宋敏[1] 黎儒青[1] 蒋天伦[1] 赵树铭[1] 

机构地区：[1]第三军医大学西南医院输血科,中国人民解放军重庆市血站,重庆400038

出　　处：《第三军医大学学报》2009年第5期409-411,共3页Journal of Third Military Medical University

基　　金：重庆市自然科学基金(2007BB5090);第三军医大学回国人员启动基金(2007XG51)~~

摘　　要：目的研究EPO对人CD34+脐血干细胞表面CD26表达的影响。方法应用流式细胞仪法检测经MACS-CD34+免疫磁珠分选并采用EPO处理后培养的人CD34+脐血干细胞,通过与酶底物反应检测人CD34+脐血造血干细胞表面CD26的表达情况。结果与对照组相比,经EPO 100 U/ml和EPO 200 U/ml 18h培养后的CD34+脐血造血干细胞群中CD26+细胞比例明显上升,其酶活性亦明显升高。结论EPO可上调CD34+脐血干细胞表面CD26的表达并增强其生物学功能。Objective To observe short-term erythropoietin （EPO） treatment on the expression of CD26, dipeptidyl-peptidase IV, in the CD34^＋ human cord blood hematopoietic ceils in vitro. Methods The CD34^＋ cells were sorted from human cord blood by magnetic activated cell sorting （MACS） technique, and subjected to recombinant human EPO treatment at a dose of 50, 100 and 200 U/ml. We detected CD26 expression of the treated and non-treated cells by flow cytometry and CD26 activity by responding with chromogenic CD26 substrate Gly-Pro-p-nitoanilide. Results CD26 expression and activity increased significantly in EPO 100 and 200 U/ml treatment group. Conclusion Short-term EPO treatment in vitro upregulates CD26 expression and activity in the CD34^＋ human cord blood hematopoietic cells, and enhances its biological function.

关 键 词：EPO CD26 脐血干细胞 

分 类 号：R329.25[医药卫生—人体解剖和组织胚胎学] R331.14[医药卫生—基础医学]