牛促卵泡激素α亚基cDNA的克隆与序列分析  

Nucleotide Sequence of Cloned cDNA for α Subunit of Bovine Follicle Stimulating Hormone

作　　者：昔奋攻[1,2] 余荭[1,2] 陈新国[1,2] 石玉瑚[1,2] 陈清轩[1,2] 张志红[1,2] 

机构地区：[1]新疆农业科学院微生物研究所 [2]中国科学院发育生物研究所

出　　处：《生物化学与生物物理进展》1998年第2期159-162,共4页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金

摘　　要：从牛脑垂体中提取总ＲＮＡ，分离ｍＲＮＡ，反转录获得ｃＤＮＡ，ＰＣＲ扩增获得长为３８０ｂｐ的牛促卵泡激素α亚基ｃＤＮＡ片段，将它克隆于ｐＵＣ１９中，进行序列分析，结果表明：所克隆的α亚基基因编码区序列与Ｅｒｗｉｎ所发表的序列基本相同，仅编码第２４位赖氨酸的密码有差异，同时将所获基因的核苷酸序列及相应氨基酸序列与人、啮齿类的同类基因相比较，具有很高的同源性．Total RNA was prepared from pituitary of bovine.mRNA was isolated from the total RNA. cDNA was synthesized as template used in PCR for amplification of cDNA for α subunit of bovine follicle stimulating hormone. Sequence analysis showed that the obtained cDNA fragment is 380 bp and all these nucleotides except one coding for Lys of No24 are as same as that Erwin reported. Comparison of bovine, human and rodent indicated that the amino acid and nucleotide sequences of the α subunit in these mammalian species are highly conservative.

关 键 词：牛促卵泡激素 促卵泡激素 Α亚基 CDNA 序列分析 

分 类 号：Q579.13[生物学—生物化学]