CS3抗原基因的表达及纤毛装配元件的研究  

Study on the Genetic Determinant for Expression and Assembly of CS3 Fimbriae

作　　者：董自正[1] 张兆山[1] 李淑琴[1] 黄翠芬[1] 

机构地区：[1]北京生物工程研究所

出　　处：《生物工程学报》1998年第2期133-138,共6页Chinese Journal of Biotechnology

基　　金：国家"863"基金

摘　　要：在肠毒素大肠杆菌（ＥＴＥＣ）的已知定居因子中，ＣＳ３是临床分离株中最常见的抗原之一。为了研究ＣＳ３纤毛装配的基本元件，绘制了ＣＳ３亚基结构基因和辅助蛋白编码区的限制酶谱。通过亚克隆的亚基基因和不同辅助蛋白基因之间的互补性表达结果，确定了ＣＳ３纤毛装配所需要的辅助蛋白的ＤＮＡ功能片段。微细胞分析结果显示，ＣＳ３基因的有效表达和纤毛的装配至少需要６条蛋白多肽分子量分别为１５ｋＤａ，１７ｋＤａ，２４ｋＤａ，２７ｋＤａ，４８ｋＤａ和９０ｋＤａ。除了１５／１７ｋＤａ的蛋白多肽为ＣＳ３亚基外，其余的蛋白多肽参与ＣＳ３亚基的转运及纤毛的装配。根据以上结果初步确定了上述相关基因的相对位置。Colonization factor antigens (CFAs) are important toxic and protective antigens of enterotoxigenic Escherichia coli (ETEC).Among the known CFAs,CS3 is a major fimbriae antigen.To study genes encoding CS3 subunit and auxillary proteins which are responsible for assembly of CS3 fimbriae,the restriction map of CS3 genetic determinant was made.Based on the map,the structural gene of CS3 subunit and the region encoding auxillary proteins were cloned respectively.Expression results of the recombinant plasmids in minicells showed that the genetic determinant of CS3 fimbriae encodes six polypeptides with the molecular weight 15,17,24,27,48 and 90kDa,respectively.Complemetary expression analysis among the different mutant showed 15kDa/17kDa proteins are CS3 subunit and it's precursor,the others may be necessary for assembly of CS3 fimbriae.The relative location of various genes of CS3 genetic determinant was determined.

关 键 词：肠毒素 大肠杆菌 表面抗原 CS3 纤毛装配 

分 类 号：Q78[生物学—分子生物学] R378.21[医药卫生—病原生物学]