外源猪生长激素基因在金鱼体内的整合与表达研究  

The Study on Integration and Expression of Porcine Growth Hormone Genein Transgenic Goldfish

作　　者：张志红[1] 刘桂生[1] 张玉廉[1] 陈清轩[1] 

机构地区：[1]中国科学院发育生物学研究所

出　　处：《生物工程学报》1998年第2期227-229,共3页Chinese Journal of Biotechnology

基　　金：国家"863"高技术项目

摘　　要：外源猪生长激素基因在金鱼体内的整合与表达研究张志红刘桂生张玉廉陈清轩（中国科学院发育生物学研究所北京１０００８０）自１９８２年Ｐａｌｍｉｔｅｒ［１］等人给小鼠受精卵雄原核注射大鼠生长激素基因培养成功“超级”鼠以来，由于人们认识到转基因技术导致动植物品...A linear recombinant plasmid, consisting of sheep metallothionein gene promoter and porcine growth hormone gene, was microinjected into goldfish fertilized eggs. The experimental goldfishes were detected and screened by using dot blotting and Southern blotting to obtain foreig gene integration positive transgenic goldfish, which were further used for mating each other. F1 and F2 generations were detected by using PCR, respectively. Our data, including PGH expression level in transgenic goldfish serum detected radioimmunoassy, showed that foreign genes have been integrated and expressed in some transgenic goldfish, and passed into next generation through sex reproduction.

关 键 词：猪生长激素 生长激素 基因整合 基因表达 金鱼 

分 类 号：Q575.11[生物学—生物化学] Q78