多重耐药鲍曼不动杆菌gyrA基因突变与环丙沙星耐药关系研究  

Mutation of gyrA gene of multi-drug resistant Acinetobacter baumannii and its resistance to ciprofloxacin

作　　者：朱健铭 吴晋兰[2] 姜如金[1] 吴康乐[1] 王建敏[1] 孔海深[3] 

机构地区：[1]杭州市余杭区中医院,浙江杭州311106 [2]杭州市余杭区第五人民医院 [3]浙江大学医学院附属第一医院

出　　处：《疾病监测》2009年第1期46-49,共4页Disease Surveillance

基　　金：杭州市余杭区重大科技项目(2008001);杭州市卫生科技计划项目(2008B0113)

摘　　要：目的研究多重耐药鲍曼不动杆菌(MDR-ABA)gyrA基因突变与环丙沙星耐药关系。方法以鲍曼不动杆菌gyrA基因序列为靶序列,用PCR+DNA测序等方法对62株临床分离株gyrA基因突变进行对比研究。结果在53株耐环丙沙星MDR-ABA菌中,有52株gyrA基因的83位表现出突变,其突变方式全为TCA→TTA,导致氨基酸丝氨酸→亮氨酸;8株环丙沙星敏感和1株环丙沙星中介的MDR-ABA菌gyrA基因无突变。结论临床分离的MDR-ABA菌对环丙沙星耐药的分子机制主要表现为gyrA基因83位氨基酸密码子突变。Objective To study the relationship between mutation of gyrA gene of multidrug resistant Acinetobacter baumannii （MDR- ABA） and the resistance to eiprofloxacin. Methods With gyrA gene sequence of ABA as target sequence, mutation of gyrA gene of 62 strains of ABA were analyzed by using PCR and DNA sequencing. Results Among 53 strains of ABA which were resistant to eiprofloxacin, 52 strains showed single point TCA→TTA mutation in the gyrA gene at codon 83. Leading to amino acid substitution of Ser→Leu. Eight strains of ABA which were sensitive to ciprofloxacin and 1 strain of ABA which was intermediary to ciprofloxacin showed no mutation. Conclusion The molecular mechanism of the resistant MDR-ABA clinically isolated was the mutation in gyrA gent at eodon 83.

关 键 词：鲍曼不动杆菌 多重耐药 GYRA基因 环丙沙星 

分 类 号：R450[医药卫生—治疗学]