守宫木致CHL及V79细胞染色体畸变作用  

Study on chromosome aberration induced by Sauropus androgynus in murine CHL and V79 in vitro

作　　者：张静[1] 熊习昆[1] 杨颖[1] 王京滨[1] 陈秀娟[1] 

机构地区：[1]广东省疾病预防控制中心毒理所/质量技术管理科,广州510300

出　　处：《中国公共卫生》2009年第3期327-329,共3页Chinese Journal of Public Health

基　　金：广东省科技计划项目(2006B20501009)

摘　　要：目的观察守宫木对体外培养细胞的遗传学毒性。方法以守宫木提取液为受试物,不同浓度条件下染毒培养中国仓鼠肺成纤维细胞(CHL)及中国仓鼠肺细胞(V79)2株细胞系,观察中期分裂相细胞的染色体畸变率。结果守宫木对CHL细胞的染色体致畸率很高(P<0.01),在50%浓度下,其直接致染色体畸变能力明显高于经S9代谢活化组,对CHL细胞的染色单体型畸变明显高于染色体型畸变。守宫木对V79细胞在50%浓度下,其直接的致染色体畸变效应不明显,经S9代谢活化,致突变效应与阴性对照组比较差异有统计学意义(P<0.05),染色单体型畸变仍高于染色体型畸变。结论守宫木具有一定的诱发染色体畸变的遗传毒性。Objective To explore the genetic toxicity of Sauropus androgynus on the chromosomal aberration in vitro. Methods Experimental liquid was extracted from Sauropus androgynus, and diluted into different concentrations. CHL and V79 cell lines were used as models and cultured with serial concentrations of Sauropus androgynus extractions. Slides were prepared and metaphase cells were observed under microscope for chromosomal aberrations. Results Sauropus androgynus caused great chromatid and chromosomal aberrations in CHL ceils （ P 〈 0. 01 ）. At the concemtration of 50% extraction, the group without S9 showed greater capacity in aberration than that of S9 treated extraction group. In CHL cells ,the chromatid aberrations were greater than chromosomal aberrations. In V79 cells, at the concentration of 50% Sauropus androgynus extraction, S9 treated group showed greater effects of aberration induction than in negative control group （ P 〈 0. 05 ）. Direct effects of Sauropus androgynus without S9 could not be seen in V79 cells and the chromatid aberration was more than chro- mosomal aberration. Conclusion Sauropus androgynus has certain genetic toxicity of chromosomal aberration induction and its molecular mechanism needs further investigation.

关 键 词：守宫木 染色体畸变 仓鼠肺成纤维细胞(CHL) V79 遗传毒性 

分 类 号：Q343.244[生物学—遗传学] R994.4[医药卫生—毒理学]