威灵仙总皂苷的分离纯化  

Separation and Purification of Total Saponins from Radix Clematidis

作　　者：黄莉[1] 黄纯兰[1] 周云[1] 李录克[1] 刘国华[2] 曾树平 

机构地区：[1]泸州医学院附属医院血液内科,四川泸州646000 [2]中国林科院林业新技术研究所,北京100091 [3]泸州维康医院,四川泸州646000

出　　处：《四川中医》2009年第3期40-42,共3页Journal of Sichuan of Traditional Chinese Medicine

基　　金：泸州市科技局科研项目资助

摘　　要：目的:研究用大孔树脂分离纯化技术从威灵仙中提取分离有效部位威灵仙总皂苷。方法:将威灵仙粉粹后用70%乙醇渗滤提取,收集浓缩提取液后,上D101型大孔树脂,依次用10%氨水、水、20%乙醇洗脱杂质,最后以70%乙醇洗脱有效部位,得总皂苷提取物,用齐墩果酸作对照,检测其含量。结果:威灵仙总皂苷主要富集于70%乙醇洗脱液部分,总皂苷含量达23.14%。结论:采用大孔吸附树脂分离纯化威灵仙总皂苷是可行的,可用于其他应用方面的研究。Objective： To establish a method for the separation and purification of total saponins from Radix Clemafidis by macroporous absorptive resin.Methods： Radix Clematidis was smashed first, extracted with 70% ethanol and purified with DI01 macroporous absorptive resin, and eluted with 10% ammonia water, distilled water, 20% ethanol and 70% ethanol.The content of total saponins was determined with triterpenoidal sapogenin-oleanolic acid as control standard. Results： Total sapcoins of Radix Clematidis were mainly enriched in the selution eluted with 70% ethanol.The yield rate was 23.14%. Conclusion： It is feasible to apply macropotous absorptive resin to isolation and purification of total saponins from Radix Clematidis.

关 键 词：威灵仙 总皂苷 大孔吸附树脂 

分 类 号：R284[医药卫生—中药学]