和中消溃汤对乙酸诱导溃疡模型大鼠EGF表达的影响  

Effect of Hezhongxiaokui Decoction on EGF Expression of Rats Gastric Ulcer Induced by Acetic Acid

作　　者：齐晶晶[1,2] 王贤斌[1] 章红波[2] 黄永章[1] 杨健业[1] 

机构地区：[1]郧阳医学院附属人民医院,十堰442000 [2]湖北中医学院,硕士生武汉430065

出　　处：《中国中医急症》2009年第3期420-422,共3页Journal of Emergency in Traditional Chinese Medicine

摘　　要：目的探讨和中消溃汤对乙酸诱导溃疡模型大鼠表皮生长因子(EGF)表达的影响。方法雄性SD大鼠随机分为正常对照组、模型组、和中消溃汤组、丽珠得乐组。除正常对照组外均用改良Okabe法造模,5d后除正常对照组正常饮食外,其余各组分别给生理盐水、和中消溃汤、丽珠得乐悬浊液灌胃,持续15d后剖腹取胃,取溃疡瘢痕部组织切片备用作免疫组化检测EGF表达水平,作HE染色及PAS染色以观察灌胃后各模型组大鼠的溃疡愈合情况,用显微镜测微尺定量测量再生黏膜厚度。结果和中消溃汤组及丽珠得乐组胃组织中EGF的表达及胃再生黏膜厚度均增加,与模型组之间具有显著差别;与丽珠得乐组相比,和中消溃汤组能显著提高胃黏膜再黏厚度及胃组织EGF表达。结论和中消溃汤能显著提高胃组织中EGF表达,其促进溃疡愈合的机制之一可能与提高胃组织中EGF表达有关。Objective： To investigate the effect of Hexhongxiaokui Decoction on EGF expression of rat gastric ulcer induced by acetic acid. Methods： 80 male SD rats were divided into 4 groups at random, named groupA（normal control group）, B （model group）, C （treated with ttexhongxiaokui Decoction）, D （treated with Lizhudele）. Except group A ,animal model of gastric ulcer in rat was set up with the modified Okabe＇ s method. 5d later, rats（groupB, C, D）had being given peroral administration of above -mentioned medicine or normal saline one time every day for 15d. 15d after medicine administration, the scars of ulcer tissue were made into slices, then examined the protein expression of EGF by immunohistochemistry. Meanwhile, after HE dyeing and PAS dyeing the slice separately were observed the healing condition of the ulcer, measured the thickness of regenerative mucosa to compare the differences between them. Results： The thickness of regenerative mucosa and the expression of mucosal EGF in group C and D was significantly increased in comparison with those in group B. Compared with group D, the thickness of regenerative mucosa and the expression of mucosal EGF in group C was significantly increased. Conclusions： Hexhongxiaokui Decoction can strengthen the expression of EGF of stomach tissue significantly. It＇ s mechanism of promoting healing of the ulcer may relate to increasing of the expression of EGF of stomach tissue.

关 键 词：和中消溃汤 改良Okabe法表 皮生长因子 大鼠 

分 类 号：R285.5[医药卫生—中药学]