shRNA表达质粒体外防治猪传染性胃肠炎病毒感染  

shRNA-expressing Plasmids Prevent Transmissible Gastroenteritis Virus Infection in vitro

作　　者：周俊芳[1,2] 华修国[1] 崔立[1] 朱建国[1] 缪德年[3] 邹勇[3] 何锡忠[3] 苏万国[3] 

机构地区：[1]上海交通大学农业与生物学院,上海200240 [2]中国水产科学研究院东海水产研究所,上海200090 [3]上海市农业科学院畜牧兽医研究所,上海201106

出　　处：《上海交通大学学报（农业科学版）》2009年第1期24-27,共4页Journal of Shanghai Jiaotong University(Agricultural Science)

摘　　要：猪传染性胃肠炎病毒是引起猪胃肠道感染的主要病原之一,新生仔猪感染后死亡率很高,给养猪业带来重大经济损失。为了探索运用RNA干扰技术防治猪传染性胃肠炎病毒感染的可能性,我们构建了两个靶向病毒基因组的小发卡RNA (shRNA)表达质粒pEGFP-U6/P1和pEGFP-U6/P2,并分别转染猪睾丸(ST)细胞。通过细胞增殖试验(MTS)和定量RT-PCR检测试验表明,这两个质粒均可高效特异地抑制猪传染性胃肠炎病毒的复制。研究结果表明,RNA干扰(RNAi)技术可以用来有效防治猪传染性胃肠炎。Porcine transmissible gastroenteritis virus （TGEV） is identified as one of the major pathogenic agents during swine entenc infection, leading to high mortality in neonatal pigs and severe annual economic loss in swine-producing areas. To investigate the possibility of using RNA interference （RNAi） as a strategy for anti-TGEV infection, two shRNA-expressing plasmids （pEGFP-U6/P1 and pEGFP-U6/P2） targeting TGEV genome were constructed and transfected into swine testicular （ST） cells. MTS assay and realtime quantitative RT-PCR both demonstrated that the two shRNA-expressing plasmids were capable of protecting ST cells from TGEV destruction with very high specificity and efficiency. Taken together, our results suggest that RNAi might become a promising new strategy for anti-TGEV infection.

关 键 词：猪传染性胃肠炎病毒 RNA干扰 SHRNA表达质粒 猪睾丸细胞 

分 类 号：S852.41[农业科学—基础兽医学]