尿中美芬妥英手性色谱分离单纯形法最优化  

Chromatographic optimization of dtiral separation of mephenytoin in urine using simplex method

作　　者：姚彤炜[1] 曾苏[1] 陈枢青[1] 阮宏强[2] 

机构地区：[1]浙江医科大学药物分析教研室,杭州310031 [2]浙江医科大学药物分析系

出　　处：《中国现代应用药学》1998年第2期37-3,共1页Chinese Journal of Modern Applied Pharmacy

基　　金：浙江省自然科学基金资助项目第396486号

摘　　要：应用单纯形法对尿中美芬妥荚(MP)的手性气相色谱分离条件进行最优化。优化结果选定柱温为185℃,载气流速为2.5ml/min。在此条件下测得柱效(以S-MP计)n=936/m,MP两对映体的分离度R为0.96％。并对20名健康志愿者口服消旋体后0～8h内的尿样进行分析测定。The gas chromatographic conditions for determining the mephenytoin enantiomers in urine using simplex method were optimized.In result,the optimum chromatographic conditions were the column temperature 185℃ and the flow of carrier 2.5ml/min.The column efficiency n = 936/m and the resolution of two enantiomers R = 0.96 was determined. Under the optimum chromatographic conditions the urine samples of 20 healthy volunteers taking 100mg mesantoin were analuzed.

关 键 词：美芬妥英 单纯形法 色谱分离 最优化 

分 类 号：R971.93[医药卫生—药品]