丹参提取物对肝癌HepG2细胞DNA去甲基化作用研究  

Study of salviae Miltiorrhize extract on DNA demethylation in HepG2 cells

作　　者：田雪飞[1] 陶一明[2] 方圆[1] 孙婧[1] 

机构地区：[1]湖南中医药大学科技处血管生物学实验室,湖南长沙410208 [2]中南大学湘雅医院肝癌研究中心,湖南长沙410008

出　　处：《湖南中医药大学学报》2009年第1期13-15,共3页Journal of Hunan University of Chinese Medicine

基　　金：湖南省自然科学基金资助项目(06JJ4054);湖南省教育厅青年资助项目(05B036)

摘　　要：目的探讨丹参提取物丹参酮ⅡA对肝癌HepG2细胞DNA甲基转移酶1基因(DNMT1)表达的抑制作用。方法采用MTT法检测丹参酮ⅡA、5-脱氧杂氮胞苷(5-Aza-cdR)对HepG2细胞的增殖抑制作用,根据回归方程求出半数抑制浓度(IC50)。以IC50含药量的丹参酮ⅡA处理肝癌HepG2细胞,并以5-Aza-cdR处理作阳性对照,处理72 h后用RT-PCR技术检测肝癌HepG2细胞DNMT1 mRNA表达水平。结果经丹参酮ⅡA处理后肝癌HepG2细胞DNMT1 mRNA表达水平明显下降,与空白对照组比较差异有统计学意义(P<0.01),但5-Aza-cdR组下降更显著,丹参酮ⅡA组与5-Aza-cdR组比较差异有统计学意义(P<0.05)。结论丹参酮ⅡA能抑制肝癌HepG2细胞DNTM1 mRNA表达,作用弱于5-Aza-cdR,DNA去甲基化作用可能是丹参酮抗肿瘤作用机制之一。Objective To investigate the inhibition of Salviae Miltiorrhize extract （Tanshinone Ⅱ A） in HepG2 cells on DNA methyhransfer 1 （DNMT1） gene expression. Methods The inhibition of Tanshinone Ⅱ A and 5-Aza-2′-deoxycytidine （5-Aza-cdR） on the proliferation of HepG2 cells were detected by MTT methods. The IC50 concentration of Tanshinone Ⅱ A was calculated according to the regression equation. HepG2 cells was selected to deal with the IC50 concentration of the extract, while the positive control with the same concentration of 5-Aza- cdR. The DNMT1 mRNA expression of HepG2 cells were detected by RT-PCR method after 72 h treatment. Results The DNMT1 mRNA expression level in HepG2 cells treated with Tanshinone Ⅱ A was significantly decreased compared to the blank control group （P〈0.01）, and that treated with 5-Aza-edR could decrease expression more significantly compared to Tanshinone Ⅱ A. The difference between Tanshinone Ⅱ A and 5-Aza-cdR was significant （P〈0.05）. Condusion Tanshinone Ⅱ A can inhibit DNMT1 mRNA expression in HepG2 cells and is less effective than 5-Aza-cdR. The role of DNA demethylation may be one of the anti-tumor mechanisms of Tanshinone.

关 键 词：丹参酮ⅡA DNA甲基转移酶 肝癌 5-脱氧杂氮胞苷 

分 类 号：R285.5[医药卫生—中药学]