寿胎丸对反复自然流产小鼠母胎界面SOCS1和SOCS3蛋白表达的影响  

Effects of Shoutai pills on protein expression of SOCS1 and SOCS3 in maternal-fetal interface of mice with recurrent spontaneous abortion

作　　者：何冬梅[1] 尤昭玲[1] 雷磊[1] 卢芳国[1] 赖毛华[1] 刘慧萍[1] 罗湘姣[1] 周薇[1] 

机构地区：[1]湖南中医药大学,湖南长沙410007

出　　处：《湖南中医药大学学报》2009年第1期26-28,共3页Journal of Hunan University of Chinese Medicine

基　　金：湖南省科技计划重点资助项目(2007FJ2005)

摘　　要：目的探讨寿胎丸对反复自然流产小鼠母胎界面SOCS1和SOCS3蛋白表达的影响。方法采用CBA/J×BALB/C建立正常妊娠小鼠模型,CBA/J×DBA/2建立反复自然流产小鼠模型,并将流产模型小鼠随机分为4组,分别为反复自然流产模型对照组及寿胎丸低、中、高剂量组,寿胎丸低、中、高剂量组从妊娠第0天开始灌胃给药,14 d后处死小鼠,取其蜕膜和胎盘组织,Western blot分别检测SOCS1和SOCS3蛋白的表达。结果寿胎丸低、中、高剂量组均可降低反复自然流产小鼠母胎界面SOCS1蛋白的表达,差异具有统计学意义(P<0.01),与正常妊娠组比较,差异无统计学意义(P>0.05);寿胎丸中、高剂量组可升高反复自然流产小鼠母胎界面SOCS3蛋白的表达,差异具有统计学意义(P<0.01～0.05),高剂量组与正常妊娠组比较,差异无统计学意义(P>0.05)。结论寿胎丸可能通过降低小鼠母胎界面SOCS1蛋白表达及提高SOCS3蛋白表达,进而调控Th细胞向Th2方向分化,故对反复自然流产有治疗作用。Objective To study the effects of Shoutai pills on the protein expression of SOCS1 and SOCS3 in maternal-fetal interface of mice with recurrent spontaneous abortion（RSA）. Methods The Normal pregnancy and RSA model were respectively induced with CBA/J×BALB/C and CBA/J×DBA/2. The mice with RSA were randomly divided into model group and low, middle and high dose groups of Shoutai pills. The mice were killed in 14 days after administration and their decidual and placental tissues were used to detect the protein expressions of SOCS1 and SOCS3 with western blotting. Results All the doses in 3 groups of Shoutai pills could de- creased the expression of SOCS1 （P〈0.01） and there was no obvious difference between normal pregnancy group and all groups of Shoutai pills（P〉0.05）. Middle and high doses of Shoutai pills could increased the expression of SOCS3 （P〈0.01-0.05） and there was no obvious differences between normal pregnancy and high dose group of Shoutai pills （P〉0.05）. Conclusion Shoutai pills can change Th cell into Th2 cell to treat RSA by decreasing the expression of SOCS1 and increasing the expression of SOCS3 in maternal-fetal interface of mice with RSA.

关 键 词：寿胎丸 反复自然流产 母胎界面 SOCS 小鼠 菟丝子 续断 桑寄生 

分 类 号：R285.5[医药卫生—中药学]