氧化低密度脂蛋白及其受体与子痫前期发病的关系  

Correlation of oxidized low-density lipoprotein and lectin-like oxidized low-density lipoprotein receptor-1 with pre-eclampsia

作　　者：张妍[1] 叶元华[1] 彭伟[1] 詹瑛[1] 

机构地区：[1]青岛大学医学院附属医院产科,266003

出　　处：《中华妇产科杂志》2009年第2期94-98,共5页Chinese Journal of Obstetrics and Gynecology

摘　　要：目的探讨氧化低密度脂蛋白（oxLDL）及血凝集素样氧化低密度脂蛋白受体1（LOX-1）与子痫前期发病的关系。方法选择2007年6月至2008年1月在青岛大学医学院附属医院产科住院分娩的子痫前期孕妇73例，其中轻度子痫前期孕妇35例（轻度子痫前期组），重度子痫前期孕妇38例（重度子痫前期组）。选取同期正常晚期妊娠妇女45例为对照组。采用酶联免疫吸附试验检测各组孕妇血浆中oxLDL水平；采用RT—PCR、蛋白印迹法分别检测各组孕妇胎盘组织中LOX—1mRNA及蛋白表达水平；采用RT—PCR检测各组孕妇胎盘组织中半胱氨酸天门冬氨酸蛋白酶-3（caspase-3）mRNA表达水平。结果（1）轻度及重度子痫前期组孕妇血浆中oxLDL水平分别为（0．42±0．11）及（0．68±0．12）mg／L，明显高于对照组的（0．35±0．14）mg／L，差异有统计学意义（P〈0．01）；重度子痫前期组又明显高于轻度子痫前期组（P〈0．01）。（2）轻度及重度子痫前期组孕妇胎盘组织中LOX，1mRNA表达水平分别为0．70±0．10及0．84±0．08，明显高于对照组的0．58±0．11，差异有统计学意义（P〈0．01）；重度子痫前期组又明显高于轻度子痫前期组（P〈O．01）。（3）轻度及重度子痫前期组孕妇胎盘组织中LOX-1蛋白表达水平分别为0．79±0．15及0．90±0．12，明显高于对照组的0．68±0．11，差异有统计学意义（P〈0．01）；重度子痫前期组又明显高于轻度子痫前期组（P〈0．01）。（4）轻度及重度子痫前期组孕妇胎盘组织中caspase-3 mRNA表达水平分别为3．82±0．18及5．39±0．14，明显高于对照组的2．19±0．20，差异有统计学意义（P〈0．01）；重度子痫前期组又明显高于轻度子痫前期组（P〈0．01）。（5）轻度及重度子痫前期组孕妇血浆中oxLDL水平与胎盘组织中LOX-1 mRNA表达水平呈正相关（r=0．93，P〈0．05）；胎盘组�Objective To investigate the role of oxidized low-density lipoprotein （oxLDL） and lectin-like oxidized low-density lipoprotein receptor-1 （LOX-1） in pre-ecalmpsia. Methods From June 2007 to January 2008,73 women with pre-eclampsia who delivered in the Department of Obstetrics, Affiliated Hospital of Qingdao University Medical College, were recruited, including 35 women with mild pre-eclampsia （MPE group） and 38 women with severe pre-eelampsia（ SPE group）. And 45 healthy pregnant women were taken as control group. Enzyme-linked immunosorbent assay （ELISA） was used to measure the plasma concentrations of oxLDL in these women. Semiquantitative RT-PCR and Western blot were used to investigate the expression of LOX-1 mRNA and protein in placenta. The expression of caspase-3 mRNA in placenta was determined using Semiquantitative RT-PCR. Results （1） The plasma concentrations of oxLDL in MPE group （ 0. 42 ± 0. 11 ） mg/L, SPE group （ 0. 68 ± 0. 12 ） mg/L, were significantly higher compared with control group （ 0. 35 ± 0. 14 ） mg/L（ P 〈 0. 01 ） and the concentrations of oxLDL in MPE group were significantly higher compared with SPE group （ P 〈 0. 01 ）. （ 2 ） The expression of LOX-1 mRNA in placenta of MPE group （0. 70 ± 0. 10） and SPE group （ 0. 84 ± 0. 08 ） were significantly higher than that in control group（0. 58 ± 0. 11 ） （ P 〈 0. 01 ） and the expression of LOX-1 mRNA in MPE group was significantly higher than that of SPE group （ P 〈 0. 01 ）. （ 3 ） The expression of LOX-1 protein in placenta of MPE group （0. 79 ±0. 15）and SPE group（0. 90 ±0. 12） were significantly higher compared with control group（0. 68 ± 0. 11） （P 〈0. 01）, while the expression of LOX-1 protein of MPE group was significantly higher compared with SPE group （ P 〈 0. 01 ）. （ d ） The expression of caspase-3 mRNA in placenta of MPE group （ 3.82 ± 0. 18） and SPE group（5.39 ±0. 14） were significantly higher than that in control group

关 键 词：先兆子痫 脂蛋白类 LDL 受体 氧化LDL 清道夫受体 E类 

分 类 号：R686[医药卫生—骨科学]