用抗SARS冠状病毒N蛋白单克隆抗体定位研究受染器官  

Study on multi-organ infection by monoclonal antibody against N protein of SARS-CoV

作　　者：戚扬 郝俊勤[2] 迟淑萍 孙艳玲 赵景民 程云 

机构地区：[1]解放军传染病研究所,北京100039 [2]解放军医学图书馆,北京100039

出　　处：《传染病信息》2009年第1期30-32,共3页Infectious Disease Information

基　　金：国家自然科学基金资助(30340010);北京市自然科学基金资助(7034050)

摘　　要：目的利用单克隆抗体研究人体内严重急性呼吸综合征(SARS)冠状病毒(SARS-CoV)感染的靶细胞。方法利用抗SARS-CoV N蛋白单克隆抗体,采用免疫组织化学检测的方法,对一例SARS死亡病例进行组织定位研究。结果在该病例多器官的组织中检测到SARS-CoV。结论SARS-CoV在体内多种组织中存在得到证实,SARS是以肺脏为主要靶器官的多脏器受染的疾病,这为SARS临床诊断和实验研究提供了依据。Objective To explore the target cells of SARS-coronavirus （CoV） infection with the involvement of monoclonal anti- body （mAb）. Methods Immunochemical staining with mAb against N protein of SARS-CoV was used to determine the infected tissues of one dead patient with SARS. Result SARS-CoV was identified in the tissues of multiple organs. Conclusions SARS-CoV is proved to be present in multiple organs and can attack multiple target cells. This offers an evidence for clinical diagnosis and experimental research, for SARS infection is multi-organ infection with lungs as its major target organ.

关 键 词：严重急性呼吸综合征冠状病毒(SARS—CoV) N蛋白 单克隆抗体 免疫组织化学 

分 类 号：R373.9[医药卫生—病原生物学]