He-Ne激光对增强UV-B辐射小麦幼苗叶绿体的影响  

Effects of He-Ne Laser on Chloroplast of Wheat Seedling Exposed to Enhanced UV-B Radiation

作　　者：郭君瑜[1] 张琴[1] 韩榕[1] 

机构地区：[1]山西师范大学生命科学学院,临汾041004

出　　处：《植物研究》2009年第2期204-208,共5页Bulletin of Botanical Research

基　　金：国家自然科学基金资助项目(30671061);山西省自然科学基金资助项目(20041101)

摘　　要：对"晋麦8号"小麦幼苗分别采用5mW·mm-2 He-Ne激光辐照、10.08kJ·m-2·d-1增强UV-B辐射及二者组合进行处理,研究各处理组小麦幼苗叶绿体膜透性、叶绿体蛋白质含量以及叶绿体偶联因子CF1的ATP酶活性、希尔反应的活性变化。结果表明:UV-B辐射后小麦幼苗叶绿体膜透性增加,叶绿体蛋白质含量有一定的下降,而ATP酶活性、希尔反应的活性均受到抑制。经过He-Ne激光辐照可使叶绿体膜透性降低、叶绿体蛋白质含量有一定的升高,同时ATP酶活性、希尔反应的活性也受到部分激活。这些变化说明增强UV-B辐射引起小麦幼苗叶绿体损伤,而一定剂量的He-Ne激光辐照可部分修复增强UV-B对小麦幼苗光合系统的损伤。The seedling of wheat （jinmai8） were exposed to He-Ne laser （5 mW · mm-2 enhanced UV-B ra- diation （ 10.08 kJ·m-2·d-1 ） and the combined treatment of He-Ne laser irradiation and enhanced UV-B radi- ation. The measurement permeability of chloroplast membrane, chloroplast protein content, Hill reaction activity and ATPase activity of wheat seedlings. Result enunciation ： the chloroplast membrane permeability has increased after UV-B radiated wheat seedlings, chloroplast protein content went down, Hill reaction activity and ATPase ac- tivity were restrained. The He-Ne laser can make chloroplast membrane permeability fall, chloroplast protein con- tent go up certainly. Hill reaction activity and ATPase activity were also partially activated. These variety explain enhanced UV-B radiation cause damage chloroplast of wheat seedling, but certain the He-Ne laser partially repair UV-B damage the system of photosynthesis on wheat seedling.

关 键 词：HE-NE激光 UV-B辐射 小麦 叶绿体 光合系统 

分 类 号：Q945.11[生物学—植物学]