干旱胁迫下沙枣和孩儿拳头叶绿素荧光特性研究  

Chlorophyll Fluorescence Characteristics of Elaeagnus angustifolia L. and Grewia biloba G.Don var.parviflora(Bge.)Hand.-Mazz.Seedlings under Drought Stress

作　　者：孙景宽[1,2] 张文辉[1] 陆兆华[2] 刘新成[1] 

机构地区：[1]天津师范大学化学与生命科学学院,天津300074 [2]滨州学院山东省黄河三角洲生态环境重点实验室,滨州256603

出　　处：《植物研究》2009年第2期216-223,共8页Bulletin of Botanical Research

基　　金：天津市科技发展项目(05YFGZNC01200);滨州学院青年人才创新工程(BZXYQNLG200721)

摘　　要：以沙枣和孩儿拳头2年生盆栽苗为材料,采用称重控水的方法设置对照(土壤含水量为25.6%～27.2%),轻度胁迫(19.2%～20.8%),中度胁迫(12.8%～14.4%),重度胁迫(6.4%～8.0%)4个梯度,研究了干旱胁迫对沙枣和孩儿拳头色素含量和叶绿素荧光特性的影响。结果表明:(1)随着干旱胁迫的加剧,两物种叶绿素a含量,叶绿素a/b比值,总叶绿素含量呈下降趋势,且叶绿素a对干旱胁迫的反应较叶绿素b敏感,但两物种叶绿素b含量和胡萝卜素含量变化规律不一致。(2)随着干旱胁迫的加剧,孩儿拳头Fm、Fv呈下降趋势,沙枣相反,但两物种F0呈增加趋势,Fv/F0、F′v/F′m呈下降趋势,Fv/Fm差异不显著;PhiPS2、ETR、qP先升高后降低,NPQ则先降低后升高。(3)虽然两物种表现出较强的抗旱性,但在重度干旱胁迫下(6.4%～8.0%),光合色素含量、叶绿素荧光参数受到较大影响,推测此时的土壤水分含量为两物种光合色素、光系统Ⅱ的耐受极限;物种间相比,孩儿拳头更为敏感,在今后的园林管护中,要根据土壤水分状况和物种间的差异做好园林灌溉。Elaeagnus angustifolia L. and Grewia biloba var. parviflora（ Bge. ）Hand.-Mazz. 2-year-old seedlings as materials,using weighing method to set up control （soil moisture 25.6% -27.2% ）, light stress （ 19.2% - 20. 8% ）, moderate stress（12.8% - 14.4% ）, severe stress（6.4% -8.0% ）, the effects of drought stress on pigment content and Chlorophyll fluorescence characteristics of E. angustifolia L. and G. biloba G. Don vat. parviflora （ Bge. ） Hand. -Mazz. seedlings were studied. The results showed ： （ 1 ） Along with the increasing degree of drought stress, the content of chlorophyll a, value of chlorophyll a/b, content of total chlorophyll of two species decreased, and the response of chlorophyll a to drought stress more is sensitive than chlorophyll b, but content of chlorophyll b and content of carotinoid of two species changed inconsistently. （2） Fro, Fv of G. biloba var. parviflo- ra （Bge.） Hand. -Mazz. decreased, E. angustifolia L. is opposite ; but F0 increased, Fv/Fo, F＇v/F＇m decreased, F,/F.1 were not significantly different, PhiPS2, ETR, qP first increased, then decreased, NPQ first decreased, then increased in two species. （3）Although the two species showed strong drought resistance, pigment content and Chlorophyll fluorescence parameters were impacted greatly in severe drought stress（6.4% -8.0% ） , so it is possible that the content of soil moisture at this point is the tolerance limit of photosynthetic pigment and PS 11 of two species, compared with E. angustifolia L. , G. biloba var. parviflora （Bge.）Hand.-Mazz. is more sensitive to drought, in the future management, garden irrigation should be guided according to the soil moisture situation and the differences between species.

关 键 词：沙枣 孩儿拳头 叶绿素荧光 干旱胁迫 

分 类 号：Q945.79[生物学—植物学]