HPLC-MS/MS法与MEIA法在监测器官移植患者他克莫司全血浓度中的比较  

Comparison on two methods of HPLC-MS/MS and MEIA in monitoring the tacrolimus concentrations for organ transplantation patients

作　　者：李鹏飞[1] 刘丽宏[1] 马萍[1] 丁春雷[1] 童卫杭[1] 

机构地区：[1]中国人民解放军第二炮兵总医院药剂科,北京100088

出　　处：《中国临床药理学与治疗学》2009年第1期80-83,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的:比较HPLC-MS/MS法与MEIA法在监测器官移植患者他克莫司全血浓度中的应用。方法:建立和确证可行的HPLC-MS/MS法,并与ME-IA法分别测定他克莫司相同样本的浓度,对测试结果进行统计分析,评价两种方法。结果:HPLC-MS/MS法测定他克莫司平均浓度为(4.86±0.46)ng/mL,MEIA法测定浓度平均为(5.52±0.43)ng/mL,两种方法测定结果相关系数r平均为0.8771,两种方法相关性较强。LC-MS/MS法测定浓度值/MEIA法测定浓度值平均值为(90.3±5.3)%。结论:HPLC-MS/MS法测定浓度为他物在体内的准确浓度,更适用于日常治疗药物监测(TDM)工作。AIM： To compare two methods of HPLC-MS/MS and MEIA to monitor the tacrolimus concentrations for organ transplantation patient. METHODS： After developing and validating the HPLC-MS/MS method to determine the concentrations of tacrolimus, the tacrolimus samples were quantitated by two methods of HPLC-MS/MS and MEIA, respectively. Evaluate two methods by statistical analysis of quantitative results. RESULTS： The mean concentration were（4.86 ±0.46） ng/mL by HPLC-MS/MS and （5.52 ± 0.43） ng/mL by MEIA. The coefficient correlation of two methods was 0. 8771, and the two methods have good correlations in statistics. The mean concentration ratio was （ 90.3 ± 5.3 ） %. CONCLUSION： The HPLC-MS/MS method is a more precise method for to determining the effective concentration of tacrolimus, which is suitable for daily TDM.

关 键 词：高效液相色谱-质谱联用法 微粒子捕捉酶免疫发光技术 器官移植 他克莫司 

分 类 号：R969[医药卫生—药理学]