水稻Ac/Ds组织培养获得再生植株突变体  

Regeneration of Rice Mutants Harbouring Ac/Ds Elements Via Tissue Culture

作　　者：袁云香[1] 万志刚[2] 孙丙耀[2] 

机构地区：[1]渭南师范学院环境与生命科学系,陕西渭南714000 [2]苏州大学基础医学与生物科学学院,江苏苏州215006

出　　处：《种子》2009年第2期9-11,共3页Seed

基　　金：国家自然科学基金项目资助(项目编号:30571049);渭南师范学院研究生科研基金项目资助(项目编号:09YKZ002)

摘　　要：用含Ac/D s转座元件的水稻种子为实验材料,从成熟胚盾片诱导愈伤组织,并经分化培养获得一定量的再生植株(R0),经田间栽培收获再生植株的成熟种子(R1)。对获得的111株再生植株(R0)叶片取样,提取基因组DNA,采用PCR方法检测其Ac/D s插入。结果表明,有94%的再生植株含有Ac/D s,6%的再生植株仅含Ac。只带有Ac而无D s的再生植株是由于D s切离而丢失,其具体机制目前还不清楚,有待进一步研究。因此,利用组织培养的方法获得更多的突变体来构建突变体库是可行的。The rice seeds, which were confirmed to harbour both Ac and Ds elements, were used as experimental materials to perform tissue culture. Regenerated plants （ R0 ） were obtained. After transfer to soil and growing,mature seeds （R1 ） were harvested from regenerated plants. Leaf sampling was carried out when the regenerated plants grew vigorously at tillering stage, and the genomic DNA extraction was conducted from these sampled leaves. The extracted genomic DNA was adopted as template in a PCR using specific primers to check the Ac/Ds insertion, Ac/Ds insertion pattern was screened by PCR from a population of 111 plants regenerated from seeds harbouring both Ac and Ds. The result showed that 94% carried both Ac and Ds elements,while 6% Ac alone. The Ac plants were devoid of Ds element, the cause of which remains unclear and needs further work. Thus, it is suggested that enlarging the mutant library via tissue culture should be feasible in rice.

关 键 词：水稻 组织培养 Ac/Ds标签系统 突变体 

分 类 号：S511[农业科学—作物学]