IGF-1对大鼠局灶性脑损伤HSP_(70)表达的影响  

Effects of IGF-1 on expression of HSP_(70) in rats following focal cerebral ischemia-reperfusion injury

作　　者：李旺辉[1] 李开荣[1] 萧洪文[1] 杨朝鲜[2] 

机构地区：[1]四川泸州医学院人体解剖教研室,泸州646000 [2]泸州医学院神经生物教研室

出　　处：《陕西医学杂志》2009年第1期11-13,19,共4页Shaanxi Medical Journal

基　　金：泸州医学院科学研究项目(泸医院科[2003]12号)

摘　　要：目的:研究胰岛素样生长因子-1(IGF-1)对大鼠局灶性脑缺血再灌注后热休克蛋白70(HSP70)表达的影响及与缺血的关系,探讨IGF-1对脑缺血再灌注损伤的保护作用。方法:制作SD大鼠大脑中动脉缺血再灌注模型。将55只SD雄性大鼠随机分为假手术组(n=5)、对照组(n=25)、IGF-1治疗组(n=25),其中后两组按缺血再灌时间(6h、12h、1d、3d、7d)不同可分为5个亚组,每组5只,治疗组于缺血2h再灌注1h后经腹腔注入40μg/kg稀释为1ml的IGF-1,假手术组及对照组同时腹腔注入生理盐水1ml。以上动物均在再灌注后规定时间点用4%多聚甲醛经心脏灌注固定,取大鼠脑组织,应用免疫组化S-P法和HE染色检测HSP70蛋白表达及脑组织结构病理变化。结果:与对照组相比,治疗组大鼠脑组织HSP70表达明显增加(P<0.05),其神经细胞坏死程度明显减轻(P<0.05)。结论:IGF-1在大鼠局灶性脑缺血再灌注损伤中起保护作用,其作用机制包括增加HSP70蛋白的表达,发挥其神经保护作用。Objective： To evaluate the protective effect of insulin-like growth factor-1 （IGF-1） on focal cerebral ischemia reperfusion in rats. Methods ：The healthy adult of male SD rats （55） were divided randomly into sham operation group（5）,model group（25） and IGF-1 group（25）, each of the model group and IGF-1 group was further divided into 5 subgroups according to the duration of reperfusion（6 hour, 12 hour, 24 hour,3 day,7 day） after two hours of cerebral ischemia. Then the rats in sham operation and model group were injected normal saline and IGF-1 group were injected IGF-1 intraperitonelly. Brain tissues of rats were used to observe nerve cell injury under light microscope after HE staining, and positive neurons of HSPT0 were observed by immunohistochemical method. Results： Compared with the model group , the injury degree of neuron greatly reduced in the IGF-1 group （-P（0. 05）, At the same time, the mumber of HSPTo positive neuron was increased significantly in IGF-1 group compared with model group （P（0. 05）. Conclusion： IGF-1 plays a protective role in rat focal cerebral ischemia-reperfusion injury, the neuroprotective mechanism of IGF-1 may involve in increasing HSP70 activity.

关 键 词：缺氧缺血 脑/预防与控制 再灌注损伤/预防与控制 胰岛素样生长因子-1/治疗应用 热休克蛋白70/代谢 模型 动物 大鼠 

分 类 号：R587.1[医药卫生—内分泌] R285.5[医药卫生—内科学]