一步法RT-PCR检测大鼠死后脑HGPRT mRNA的降解  

Detection of brain HGPRT mRNA degradation in rats by one-step RT-PCR

作　　者：朱方成[1] 雷怀成[1] 

机构地区：[1]郧阳医学院法医学教研室,十堰442000

出　　处：《陕西医学杂志》2009年第1期14-16,共3页Shaanxi Medical Journal

基　　金：郧阳医学院研究生启动基金资助计划项目(No2007QDJ11)

摘　　要：目的:探讨大鼠死后不同时间次黄嘌呤鸟嘌呤磷酸核糖转移酶(HGPRT)mRNA的降解情况与死亡时间(PMI)的关系。方法:将36只大鼠断颈处死后置于20℃温度控制系统内,利用一步法荧光标记RT-PCR技术检测大鼠脑管家基因HGPRT mRNA在死后即刻至8d的相对表达量的变化。结果:在死后即刻至7d大鼠脑组织均可检测到HGPRT mRNA,其扩增产物呈下降趋势。结论:一步法RT-PCR技术检测大鼠脑组织HGPRT mRNA,在死后的降解呈现出一定的规律性,可为PMI推断提供一种新的指标。Objective：To explore the relationship between postmortem interval （PMI） and timedependent degradation of Hypoxanthine guanine phosporibosyltransferase（HGPRT） mRNA in the brain of rat. Methods ： 36 rats were killed by cervical dislocation and placed in a chamber with temperature-controlling system at 20℃ for 0 to 8d postmortem. Changes of house-keeping HGPRT mRNA in brain were examined by one-step fluorimetric reverse transcriptase-polymerase chain reaction（RT-PCR）. Results ：The amplification production of HGPRT mRNA could be detected in brain within 7d postmortem, which showed a decreasing tendency. Conclusion：Time-dependent degradation of HGPRT mRNA in brain detected by one-step fluorimetric RT-PCR technique may provide a new indicator for estimation of PMI.

关 键 词：死亡/诊断 次黄嘌呤鸟嘌呤磷酸核糖转移酶/代谢 逆转录-聚合酶联反应 动物 实验 大鼠 

分 类 号：R373.1[医药卫生—病原生物学] R827.14[医药卫生—基础医学]