壳寡糖抑制肝癌细胞SMMC-7721的增殖及其机制探讨  

Studies on the Inhibitory Effects of Oligochitosan on SMMC-7721 Cells of Hepatocarcinoma

作　　者：许青松[1,2] 魏鹏[1,2] 窦江丽[1,2] 麻攀[1,2] 白雪芳[1] 杜昱光[1] 

机构地区：[1]中国科学院大连化学物理研究所天然产物与糖工程组,大连116023 [2]中国科学院研究生院,北京100049

出　　处：《天然产物研究与开发》2009年第1期152-154,共3页Natural Product Research and Development

摘　　要：探讨壳寡糖抑制人肝癌细胞株SMMC-7721的增殖作用及其分子机制。采用噻唑蓝(MTT)法检测壳寡糖的细胞增殖抑制作用;利用流式细胞仪检测肝癌细胞的凋亡情况;用RT-PCR和Western blot方法研究壳寡糖引起凋亡的原因。结果显示,0.8mg/mL的壳寡糖能够抑制SMMC-7721细胞增殖,并且促进SMMC-7721细胞的凋亡,其原因是壳寡糖能够上调促凋亡蛋白Bax的表达。The activity and mechanism of oligochitosan against human hepatocellular carcinoma ceils （SMMC-7721 cells） were investigated in this study. MTT assay was used for evaluation of proliferation of SMMC-7721 ;Apoptotic cells were detected by flow cytometry using propidium iedide;RT-PCR and Western blot were used for explaining the apopto- sis of SMMC-7721. The results showed that oligochitosan notably inhibited the SMMC-7721 cells proliferation and induced the apoptosis of them. The mechanism was possibly that oligochitosan up-regulated pro-apoptotic gene Bax, and triggered the cells a start-up of the apoptosis program.

关 键 词：壳寡糖 肝癌 SMMC-7721细胞 凋亡 BAX蛋白 

分 类 号：Q53[生物学—生物化学] R256.4[医药卫生—中医内科学]