乙肝病毒血清标志物与PCR检测HBV—DNA的关系  

Relationship Between Seral Markers of Infection with HBV and the Detection of HBV-DNA by PCR Technique

作　　者：申燕[1] 艾平[1] 高军[1] 

机构地区：[1]包头医学院肝病研究室,包头014010

出　　处：《包头医学院学报》1998年第1期27-29,共3页Journal of Baotou Medical College

摘　　要：为了探讨PCR技术在乙肝病毒感染诊断中的应用及HBV—NDA与HBVM间的关系，对524例乙肝病毒感染者的血清采用PCR与ELIAS进行检测。结果：HBsAg、抗—HBc、HBeAg三项阳性组，HBVM全阴组，单项抗—HBs阳性组，HBV—DNA检出率分别为80．14％（131／141）、47．62％（20／42）、26．47％（9／34）。提示三项阳性组HBV—DNA检出率是8组形式中的最高组，标志病毒复制活跃、传染性强。HBVM全阴组也不能排除乙肝病毒感染的可能性。单项抗—HBs阳性仍有慢性肝炎存在的可能。故PCR检测HBV—DNA丰富和更新了对HBV感染的认识。但是表达非复制状态的HBV感染ELISA检测，HBVM作为初筛还是不可缺少的。To invertigate the possible application with hepatitis type B viruo as well as the relationship between HBV-DNA and HBVM,the serum of 524 patients infected with hepatitis typeB virus was detcted by using PCR and ELIAS techmques. The results indicate that the detection rates are 80.14% (131/141), 47.6% (20/42) and 26.47% (9/34) respectively in Group Awhere HBsAg, anti-HBC, and HBeAg are all positive; Group B where HBVM is negativeand Group C where only anti-HBs is positive it is suggested that the highest detection rate ofHBV-DNA falls into Group A, indicating that the duplication of the virus in active andhighly infections Negative HBVM in Group B does not rule out the possibility of infectionwith hepatitis Type-B virus. It is possible that there exists chronic hepatitis in Group CPCR delection of HBV-DNA enriches nad renew the knowledge of infection with HBV.However, ELISA detection of HBVM as initial screening is indispensible for expressing thenon-duplicating state of HBV

关 键 词：聚合酶链反应 HBV-DNA 血清标志 乙型肝炎 

分 类 号：R512.620.4[医药卫生—内科学]