不同来源的金黄色葡萄球菌耐药率比较分析  

Antimicrobial-resistant Profile of Staphylococcus aureus from Different Origins

作　　者：陈坚[1] 王彩虹[2] 王金果[1] 余方友[1] 

机构地区：[1]温州医学院附属第一医院实验诊断中心,浙江温州325000 [2]温州医学院生命科学学院、检验医学院,浙江温州325000

出　　处：《中华医院感染学杂志》2009年第5期571-573,共3页Chinese Journal of Nosocomiology

摘　　要：目的探讨不同标本来源的金黄色葡萄球菌耐药谱。方法采用常规方法分离金黄色葡萄球菌,用全自动微生物分析仪进行菌种鉴定和药敏试验,采用苯唑西林纸片扩散法检测耐甲氧西林金黄色葡萄球菌(MRSA),统计分析用χ2检验。结果分离金黄色葡萄球菌248株,标本随机挑取自血液、导管、脓液、痰液、尿液,分别为25、27、89、93、14株;所有分离株对呋喃妥因和万古霉素敏感,脓液分离的金黄色葡萄球菌对氯霉素、庆大霉素、苯唑西林、青霉素、利福平、四环素、左氧氟沙星的耐药率显著低于痰液分离株的耐药率(P<0.05);MRSA的总分离率为68.0%,脓液分离的MRSA对氯霉素、庆大霉素、青霉素、利福平、四环素、左氧氟沙星的耐药率显著低于痰液分离株的耐药率(P<0.05)。结论脓液分离的金黄色葡萄球菌和MRSA耐药率普遍低于痰液分离株的耐药率。OBJECTIVE To investigate the antimicrobial-resistance of Staphylococcus aureus isolates which obtained from different origins. METHODS The isolates of S. aureus were derived from clinical samples by routine methods. The identification and susceptibility test of the isolates were determined by Automated Microbiology Analyzer. Disk-diffusion test （K-B method） was used for detecting meticillin-resistant S. aureus （MRSA）. Х^2 Test was made to identify the significance of difference. RESULTS A total of 248 S. aureus strains were isolated from blood （25）, medical internal catheter （27）, pus （89）, sputum （93）, and urine （14）. All isolates of S. aureus were sensitive to nitrofurantoin and vancomycin. The resistant rates of S. aureus isolates from pus to chloramphenicoi, gentamicin, oxaeillin, penicillin, rifampicin, tetracycline and levofloxacin all significantly lower than those of isolates from sputum （P〈0.05）. 68.0% Of isolates were referred as MRSA. The resistant rates of MRSA from pus to chloramphenicol, gentamiein, penicillin, rifampicin, tetracycline and levofloxacin all significantly lower than those of isolates from sputum （P〈0.05）. CONCLUSIONS A high isolation rate of S. aureus and MRSA from sputum was observed.

关 键 词：金黄色葡萄球菌 耐甲氧西林金黄色葡萄球菌 纸片扩散法 耐药率 

分 类 号：R978.1[医药卫生—药品]