美罗培南对lasR／rhlR基因缺陷型铜绿假单胞菌早期生物膜的作用  

Effect of Meropenem on Biofilms of Pseudomonas aeruginosa lasR/rhlR Mutations in vitro

作　　者：刘晓岚[1] 宋志军[2] 王飞[3] 吴红 陈一强[5] 王立赞[1] 孔晋亮[5] 王恒壮[5] 

机构地区：[1]济宁医学院病理生理学教研室,山东济宁272013 [2]广西医科大学病理生理学教研室,广西南宁530021 [3]山东省文登整骨医院外科,山东文登264400 [4]丹麦国家医院临床微生物科,丹麦哥本哈根 [5]广西医科大学一附院呼吸内科,广西南宁530023

出　　处：《中华医院感染学杂志》2009年第6期604-607,共4页Chinese Journal of Nosocomiology

基　　金：国家自然科学基金资助项目(30760084);广西壮族自治区卫生厅青年基金资助项目(Z2007048)

摘　　要：目的研究美罗培南对铜绿假单胞菌PAEO1野生型及其PAEO1 lasR、PAEO1 rhlR、PAEO1 lasR rhlR基因缺陷型菌株早期生物膜作用的差异。方法采用生理盐水-吸痰管系统进行铜绿假单胞菌PAEO1野生型、PAEO1 lasR、PAEO1 rhlR、PAEO1 lasRrhlR基因缺陷型菌株体外生物膜的培养,3 d后以26μg/ml的美罗培南溶液作用24 h,用扫描电子显微镜观察生物膜的情况。结果3 d后PAEO1野生型可形成较厚、有孔状通道的成熟生物膜结构,PAEO1 lasR、PAEO1 rhlR、PAEO1 lasRrhlR基因缺陷型黏附、聚集形成明显稀薄的早期生物膜结构;经过26μg/ml的美罗培南溶液作用24 h后,PAEO1野生型的生物膜结构明显稀薄,PAEO1 lasR、PAEO1 rhlR和PAEO1 lasRrhlR基因缺陷型的生物膜结构基本被破坏,其余下散在碎片状结构。结论lasR/rhlR基因可影响铜绿假单胞菌早期生物膜的形成及其对美罗培南的敏感性。OBJECTIVE To understand the effects of meropenem on the biofilms of Pseudomonas aeruginosa lasR/ rhlR mutations in vitro. METHODS The biofilms of PAEO1 and its lasR, rhlR, and lasR rhlR mutants were incubated in a polyvinyl chloride tube in 0.9% saline for three days,then were immersed in meropenem solution of 26 μg/ml for 24 hours, and examined by scanning electron microscopy （SEM）. RESULTS After three days incubation, PAEO1 biofilms showed a well-developed structure, however, the biofilms of PAEO1 lasR, rhlR, and lasR rhlR mutants were thin and poor developed; after 24 hours in meropenem solution, PAEO1 biofilm become rareness, whereas PAEO1 lasR, rhlR, and lasR rhlR mutants biofilms were almost destructed and only small pieces left. CONCLUSIONS lasR/rhlR Genes play probably an important role in the formation of P. aeruginosa biofilm and in the resistance to meropenem.

关 键 词：生物膜 群体感应 铜绿假单胞菌 美罗培南 

分 类 号：R978.1[医药卫生—药品]