局部抗菌药物载药系统克林霉素磷酸钙水泥的实验研究  

Clindamycin-loaded Calcium Phosphate Cement as Local Antibiotic Delivery System

作　　者：储诚兵[1] 黎檀实[1] 郝立波[2] 王慧[3] 曹敬荣[4] 王继芳[2] 

机构地区：[1]解放军总医院急诊科,北京100853 [2]解放军总医院骨科,北京100853 [3]清华大学材料系,北京100084 [4]解放军总医院微生物科,北京100853

出　　处：《中华医院感染学杂志》2009年第6期625-628,共4页Chinese Journal of Nosocomiology

基　　金：国家自然科学基金项目(39670731)

摘　　要：目的探讨磷酸钙水泥(CPC)复合克林霉素制成局部抗菌药物载药系统的可行性。方法观察CPC复合2%、5%克林霉素后,对初凝时间(tI)及终凝时间(tF)的影响;高效液相色谱法(HPLC)测定2%、5%复合克林霉素磷酸钙水泥(CLCPC)试样浸于PBS液中每日释放克林霉素的浓度及持续时间;采用平皿扩散法观察2%、5%CLCPC及2%复合克林霉素Palacos骨水泥(CLPMMP)体外抑菌能力;X射线晶体衍射(XRD)分析及扫描电镜(SEM)观察克林霉素对CPC固化产物及结构有无影响。结果克林霉素缩短了2%、5%CLCPC的固化时间;2%5、%CLCPC释放克林霉素在最初6 h呈暴发性,到第4天克林霉素释放速度减慢,至42 d仍能释放克林霉素;2%CLPMMP比2%CLCPC的每日抑菌环小,2%CLCPC又比5%CLCPC的抑菌环小;至抑菌试验42 d,CLCPC及CLPMMP仍均有抑菌作用;从抑菌试验30 d起,放置2%CLPMMP试样处可见大量细菌菌落,而2%、5%CLCPC无此现象;XRD分析及SEM观察克林霉素对CPC固化产物、晶体大小及结构无影响。结论克林霉素可复合CPC制成局部抗菌药物载药系统。OBJECTIVE To determine the feasibility of clindamycin-loaded calcium phosphate cement （CLCPC） as a local antibiotic delivery system. METHODS The initial setting time （tI） and the final setting time （tF） were measured for 0%, 2% and 5% CLCPC according to ASTM C266-89 method. Clindamycin concentrations eluting from the samples of 2% and 5% CLCPC in PBS were analyzed by HPLC at different times. The bacteriostasis tests were done by plate diffusion method for 2% and 5% CLCPC and 2% Palaeos R-40 bone cement （PMMP） samples, and the diameters of the bacteriostasis ring and bacteriostasis duration were observed. The setting product and crystal size of 0% ,2% and 5% CLCPC were analyzed and observed by X-ray diffraction （XRD） and scanning electron microscopy （SEM）. RESULTS The setting time could be shortened by adding clindamycin （tI, tF） of 2% and 5 % CLCPC. Clindamycin was with burst-release from CLCPC within the intial 6-hour period and the release rate slowed down on 4th day. Clindamycin could still release until to the 42th day. The ring of 2% Palacos R-40 bone cement （PMMP） bacteriostasis was smaller than that of 2% CLCPC, and the ring of 2% CLCPC bacteriostasis was smaller than that of 5%CLCPC. The bacteriostasis still existed to the 42th day of test for 2% and 5%CLCPC and 2% Clindamycin-loaded Palacos R-40 bone cement （CLPMMP）. From the 30th day, many bacterial colonies were seen in the culture media laying 2% CLPMMP sample. On the contrary, bacterial colony was not found in the media putting 2% and 5%CLCPC. XRD and SEM showed that clindamycin did＇t have an influence on setting product, crystal size and structure of CPC. CONCLUSIONS Clindamycin-loaded calcium phosphate cement can be used as a local antibiotic delivery system.

关 键 词：磷酸钙水泥 克林霉素 抗菌药物 载药系统 

分 类 号：R378.1[医药卫生—病原生物学]