杀菌/渗透增强蛋白mRNA在大鼠体内的分布  

Distribution of Bactericidal/permeability Increasing Protein mRNA in Rat

作　　者：王昕昕[1] 梁宁生[1] 

机构地区：[1]广西医科大学附属肿瘤医院药剂科,广西南宁530021

出　　处：《中华医院感染学杂志》2009年第6期629-631,共3页Chinese Journal of Nosocomiology

基　　金：广西科学基金资助项目(0639026);广西医疗卫生重点科研课题(重200707)

摘　　要：目的研究杀菌/渗透增强蛋白(BPI)mRNA在正常大鼠体内的存在情况,为了解和临床应用BPI提供重要的信息。方法从大鼠不同器官和组织的匀浆中提取总RNA,合成第1链cDNA,利用PCR扩增大鼠BPI的DNA片段。结果遍及全身的21种器官和组织中,肾、卵巢、睾丸、肝脏、小肠、大肠、胸腺、脾脏和白细胞等9种器官中均能检测到BPI的mRNA,其中,在睾丸中最为丰富,在肝脏、大肠中也较为丰富。结论BPI的mRNA在正常大鼠体内多个器官和组织中都存在,提示它在机体内控制细菌感染的作用较为广泛。OBJECTIVE To investigate systematically the existence of bactericidal/permeability increasing protein （BPI） in normal rat organs and tissues, for providing important information about BPI in clinical application. METHODS The total RNA was extracted from organs and tissues homogenates. Then the first-strand cDNA was synthesized and BPI DNA was amplified by PCR. RESULTS BPI mRNA was found in kidneys, ovary, testes, liver, small intestine, large intestine, thymus, spleen and neutrophils of 21 various organs and tissues. Among them,BPI mRNA content was the richest in testes, relatively rich in liver and large intestine. CONCLUSIONS BPI mRNA presents in many organs and tissues of rat and it indicates that the role of BPI in host defense against bacterial infection is relatively widespread.

关 键 词：大鼠 杀菌/渗透增强蛋白 信使核糖核酸 分布 

分 类 号：R446.62[医药卫生—诊断学]