鲍氏不动杆菌医院感染株亲缘性分析3种方法学的比较  

Comparison of Three Methods on Relatedness Analysis of Acinetobacter baumannii Strains from Hospital Infection

作　　者：许小敏[1] 陈琳[1] 糜祖煌[2] 金辉[3] 张顺[1] 

机构地区：[1]宁波市第二医院检验科,浙江宁波315010 [2]无锡市克隆遗传技术研究所,江苏无锡214026 [3]东南大学公共卫生学院流行病学与卫生统计学系,江苏南京210009

出　　处：《中华医院感染学杂志》2009年第6期713-716,共4页Chinese Journal of Nosocomiology

基　　金：宁波市自然基金(2008A610090)

摘　　要：目的鲍氏不动杆菌(ABA)医院感染株亲缘性3种分析方法学的比较。方法分别采用脉冲场凝胶电泳(PFGE),扩增片段长度多态性分析(AFLP)和多基因聚类分析对27株鲍氏不动杆菌临床分离株进行分析。结果PFGE分析显示其中的19株鲍氏不动杆菌为同一克隆传播;AFLP显示24株鲍氏不动杆菌为同一克隆传播;而多基因聚类分析则显示,携带8种基因(TEM、OXA-23群、ADC、aac(3)-Ⅰ、aac(6′)-Ⅰ、ant(3″)-Ⅰ、qacE△1-sul1i、ntⅠ1)、携带7种基因(TEM、ADC、aac(3)-Ⅰ、aac(6′)-Ⅰ、ant(3″)-Ⅰ、qacE△1-sul1i、ntⅠ1)、携带6种基因(TEM、ADC、aac(3)-Ⅰ、ant(3″)-Ⅰ、qacE△1-sul1i、ntⅠ1)的3种ABA克隆为医院的流行株(3个克隆)。结论鲍氏不动杆菌医院感染株亲缘性分析中多基因聚类分析法分辨率高于PFGE与AFLP法。OBJECTIVE To compare the three methods on relatedness analysis of Acinetobacter baurnannii strains in hospital infection. METHODS Twenty-seven A. baumannii strains caused hospital infection were analyzed by pulsed field gel electrophoresis （PFGE）, amplified fragment length polymorphism （AFLP） and multiple gene cluster analysis. RESULTS PFGE analysis showed 19 strains isolated from clinic were with identical clone; AFLP analysis showed 19 strains isolated from clinic were with identical clone; but multiple gene cluster analysis showed 3 clones including 11 strains carried with 8 positive genes （TEM, OXA-23group, ADC, aac（3）-Ⅰ , aac（6′）-Ⅰ , ant（3″）-I , qacE△1-sul1 and int Ⅰ1）, 6 strains with 7 positive genes （TEM, ADC, aac（3）-Ⅰ , aac（6′）-Ⅰ , ant （3″）- Ⅰ , qacE△1 sull and int Ⅰ 1） and 4 strains with 6 positive genes （TEM, ADC, aac（3） Ⅰ , ant（3″）-Ⅰ , qacE △1-sul1 and int Ⅰ 1）. CONCLUSIONS The resolving ability of multiple gene cluster analysis is higher than that of PFGE and AFLP in relatedness analysis of A. baumannii strains from hospital infection.

关 键 词：鲍氏不动杆菌 脉冲场凝胶电泳 扩增片段长度多态性分析 多基因聚类分析 

分 类 号：R378[医药卫生—病原生物学]