大肠杆菌氯霉素类耐药基因三重PCR检测试剂盒的研究与应用  

Development of multiplex PCR detecting kit and its applicaton to the detection of chloramphenicols resistant genes in Escherichia coli

作　　者：杨鑫[1] 王红宁[1] 张安云[1] 夏青青[1] 曾博[1] 

机构地区：[1]四川大学生命科学学院动物疾病防控生物工程研究中心,四川成都610064

出　　处：《中国兽医杂志》2009年第2期11-13,共3页Chinese Journal of Veterinary Medicine

基　　金：国家科技支撑计划"规模化猪场和养殖小区生物安全控制技术研究与示范"(2007BAD51B05);"荣昌猪健康养殖生物安全控制技术研究与示范"(2006BAD14B05-05)

摘　　要：本研究研制了大肠杆菌氯霉素类药物耐药基因cat1、flor、cmlA基因三重PCR检测试剂盒。结果显示,该试剂盒具有良好的重复性、灵敏度以及保存期长等特点。应用该试剂盒检测107株猪源鸡源大肠杆菌,cat1、flor、cmlA三种基因的检出率分别是41.1%、51.4%和36.4%。本试剂盒对氯霉素类药物耐药基因的传播、流行调查以及在临床用药方面具有重要的指导意义。We developed a multiplex PCR detecting kit for the detection of chloramphenicols resistant genes cat1 ,flor and cmlA ,which was reproductive, sensitive and long-time saved. 107 strains of Escherichia coli were detected by the kit , and the detection frequencies of cat1 , flor and crnlA was 41. 1%, 51.4% and 36.4% ,respectively. The kit is of significance to the detection of the prevalence of chloramphenicol resistance genes, which can help to determine the therapeutic and prophylactic strategies in veterinary medicine.

关 键 词：大肠杆菌 三重PCR 耐药基因 氯霉素类 试剂盒 

分 类 号：S855.12[农业科学—临床兽医学]