复方五仁醇胶囊含药血清中五味子醇甲、五味子醇乙、五味子甲素、五味子乙素的测定  

Determination of schisandrin,schisandrol B,deoxyschizandrin,and schisandrin B in serum containing drug of Compound Wurenchun Capsula

作　　者：窦志华[1] 丁安伟[2] 罗琳[3] 王陆军[1] 张兵[1] 

机构地区：[1]南通大学附属南通第三医院,江苏南通226006 [2]南京中医药大学,江苏南京210029 [3]南通大学,江苏南通226001

出　　处：《中草药》2009年第3期386-389,共4页Chinese Traditional and Herbal Drugs

基　　金：江苏省中医药局科研项目(H05173);江苏省高校研究生科技创新计划项目(140)

摘　　要：目的建立复方五仁醇胶囊含药血清中五味子醇甲、五味子醇乙、五味子甲素和五味子乙素的测定方法。方法采用高效液相色谱法。色谱条件：Lichrosphere C18色谱柱（250mm×4．6mm，5μm），Phenomenex@ Description C18保护柱（4．0mm×3．0mm）；水-乙腈梯度洗脱；体积流量1．0mL／min；柱温30℃；检测波长210nm。结果五味子醇甲、五味子醇乙、五味子甲素和五味子乙素分别在0．0512～0．7680μg（r=0．9995）、0．0540～0．8100μg（r=0．9996）、0．0123～0．1845μg（r=0．9998）、0．0398～0．5970μg（r=0．9996）线性关系良好，含药血清中4种成分的平均质量浓度分别为8．0211、6．2310、0．5308、5．8510μg／mL。结论该法简便、准确、专属性强，可用于复方五仁醇胶囊含药血清中木脂素类成分的测定。Objective To determine schisandrin, schisandrol B, deoxyschizandrin, and schisandrin B in serum containing drug of Compound Wurenchun Capsula. Methods An HPLC method was set up. Lichrosphere C18 column （250 mm ×4. 6 mm, 5 μm） and Phenomenex Description C18 （4.0 mm×3.0 mm）protective column were used. Acetonitrile-water was used as gradient mobile phase. The flow rate was 1.0 mL/min. The column temperature was 30 ℃ and the detection wavelength was 210 nm. Results The linear ranges of schisandrin, schisandrol B, deoxysehizandrin, and schisandrin B were within 0. 051 2- 0. 768 0 μg （r=0. 999 5）, 0. 054 0-0. 810 0 μg （r=0. 999 6）, 0. 012 3-0. 184 5 μg （r=0. 999 8） , and 0. 039 8-0. 597 0 μg （r=0. 999 6）, respectively. The average concentration of these four lignans in serum containing drug were 8. 021 1, 6. 231 0, 0. 530 8, and 5. 851 0 μg/mL, respectively. Conclusion This method is easy, sensitive, specific, and accurate for the assaying of the four lignans in serum containing drug of Compound Wurenchun Capsula.

关 键 词：复方五仁醇胶囊 含药血清 五味子醇甲 五味子醇乙 五味子甲素 五味子乙素 高效液相色谱 

分 类 号：R286.02[医药卫生—中药学]